CONDITIONS ENHANCING PERCENTAGE AND SIZE OF E-ROSETTES FORMED BY T-LYMPHOCYTES

Three enhancing conditions of the E rosette test were studied alone and in combination to determine their effect on the total percentage and the size of rosettes formed. The three conditions studied were: (1) HABS, (2) 4°C incubation, and (3) AET pretreatment of sheep erythrocytes. AET pretreatment of E brought about the most striking enhancement of total percentage of rosettes formed. All three conditions affected rosette size causing an increase in the percentage of large rosettes with a concomitant decrease in small rosettes. When enhancing conditions were combined, an additive and predictable increase in the percentage of large morula rosettes was evident suggesting different mechanisms of enhancement are involved. © 1978.