A NEW SYSTEM FOR THE EXPRESSION OF HUMAN INTERLEUKIN-6 IN ESCHERICHIA-COLI

被引：27

作者：

YASUKAWA, K

SAITO, T

机构：

[1] Biotechnology Research Laboratory, TOSOH Corporation, Ayase-shi, Kanagawa-ken, 252, 2743-1, Hayakawa

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 06期

关键词：

D O I：

10.1007/BF01024396

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We constructed a new type of plasmid from which peptides containing interleukin-6 (IL-6) were produced in E. coli as human growth hormone (hGH) fusion proteins. The products were cleaved by thrombin to liberate IL-6, which was purified to homogeneity. © 1990 Kluwer Academic Publishers.

引用

页码：419 / 424

页数：6

共 11 条

[1] HUMAN B-CELL STIMULATORY FACTOR-II EXPRESSED IN ESCHERICHIA-COLI [J].

ASAGOE, Y ;

YASUKAWA, K ;

SAITO, T ;

MARUO, N ;

MIYATA, K ;

KONO, T ;

MIYAKE, T ;

KATO, T ;

KAKIDANI, H ;

MITANI, M .

BIO-TECHNOLOGY, 1988, 6 (07) :806-809

[2] COMPLEMENTARY-DNA FOR A NOVEL HUMAN INTERLEUKIN (BSF-2) THAT INDUCES LYMPHOCYTES-B TO PRODUCE IMMUNOGLOBULIN [J].

HIRANO, T ;

YASUKAWA, K ;

HARADA, H ;

TAGA, T ;

WATANABE, Y ;

MATSUDA, T ;

KASHIWAMURA, S ;

NAKAJIMA, K ;

KOYAMA, K ;

IWAMATSU, A ;

TSUNASAWA, S ;

SAKIYAMA, F ;

MATSUI, H ;

TAKAHARA, Y ;

TANIGUCHI, T ;

KISHIMOTO, T .

NATURE, 1986, 324 (6092) :73-76

[3] SYNTHESIS OF A GENE FOR HUMAN GROWTH-HORMONE AND ITS EXPRESSION IN ESCHERICHIA-COLI [J].

IKEHARA, M ;

OHTSUKA, E ;

TOKUNAGA, T ;

TANIYAMA, Y ;

IWAI, S ;

KITANO, K ;

MIYAMOTO, S ;

OHGI, T ;

SAKURAGAWA, Y ;

FUJIYAMA, K ;

IKARI, T ;

KOBAYASHI, M ;

MIYAKE, T ;

SHIBAHARA, S ;

ONO, A ;

UEDA, T ;

TANAKA, T ;

BABA, H ;

MIKI, T ;

SAKURAI, A ;

OISHI, T ;

CHISAKA, O ;

MATSUBARA, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (19) :5956-5960

[4]

Jones AS, 1960, COMPREHENSIVE ANAL C, VI, P495

[5] THE BIOLOGY OF INTERLEUKIN-6 [J].

KISHIMOTO, T .

BLOOD, 1989, 74 (01) :1-10

[6] ESTABLISHMENT OF AN INTERLEUKIN 6 (IL 6)/B-CELL STIMULATORY FACTOR 2-DEPENDENT CELL-LINE AND PREPARATION OF ANTI-IL-6 MONOCLONAL-ANTIBODIES [J].

MATSUDA, T ;

HIRANO, T ;

KISHIMOTO, T .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (06) :951-956

[7] OVERPRODUCTION OF HUMAN INSULIN-LIKE GROWTH FACTOR-II IN ESCHERICHIA-COLI [J].

MISOKA, F ;

MIYAKE, T ;

MIYOSHI, K ;

SUGIYAMA, M ;

SAKAMOTO, S ;

FUWA, T .

BIOTECHNOLOGY LETTERS, 1989, 11 (12) :839-844

[8] EFFICIENT CLEAVAGE BY ALPHA-THROMBIN OF A RECOMBINANT FUSED PROTEIN WHICH CONTAINS INSULIN-LIKE GROWTH FACTOR-I [J].

NISHIKAWA, S ;

YANASE, K ;

TOKUNAGADOI, T ;

KODAMA, K ;

GOMI, H ;

UESUGI, S ;

OHTSUKA, E ;

KATO, Y ;

SUZUKI, F ;

IKEHARA, M .

PROTEIN ENGINEERING, 1987, 1 (06) :487-492

[9]

SUZUKI H, UNPUB BIOCH BIOPHYS

[10] HIGH-LEVEL EXPRESSION OF HUMAN BSF-2/IL-6 CDNA IN ESCHERICHIA-COLI USING A NEW TYPE OF EXPRESSION-PREPARATION SYSTEM [J].

TONOUCHI, N ;

OOUCHI, N ;

KASHIMA, N ;

KAWAI, M ;

NAGASE, K ;

OKANO, A ;

MATSUI, H ;

YAMADA, K ;

HIRANO, T ;

KISHIMOTO, T .

JOURNAL OF BIOCHEMISTRY, 1988, 104 (01) :30-34

← 1 2 →