INFLUENCE OF PH ON THE CONFORMATION AND STABILITY OF MISMATCH BASE-PAIRS IN DNA

被引：111

作者：

BROWN, T ^{[1
]}

LEONARD, GA ^{[1
]}

BOOTH, ED ^{[1
]}

KNEALE, G ^{[1
]}

机构：

[1] PORTSMOUTH POLYTECH,SCH BIOL SCI,BIOPHYS LABS,PORTSMOUTH PO1 2DT,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 212卷 / 03期

关键词：

D O I：

10.1016/0022-2836(90)90320-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of self-complementary dodecanucleotide duplexes containing two symmetrically disposed mismatches have been studied by pH-dependent, ultraviolet light melting techniques. The results indicate that A · C, and C · C mismatches are strongly stabilized by protonation and that the degree of stabilization of the A · C mismatch depends greatly on the flanking bases. In one case, a duplex containing two A · C mismatches is more stable than the native sequence below pH 5.5. The G · A mismatch displays conformational flexibility, with a protonated G(syn) · A(anti) base-pair occurring in certain base stacking environments but not in others. The A · A and T · C mismatches are not stabilized at low pH. These solution studies correlate well with predictions based on X-ray crystallographic data. © 1990.

引用

页码：437 / 440

页数：4

共 18 条

[1] CYTIDINIUM-CYTIDINE COMPLEX - INFRARED AND RAMAN SPECTROSCOPIC STUDIES [J].

BORAH, B ;

WOOD, JL .

JOURNAL OF MOLECULAR STRUCTURE, 1976, 30 (01) :13-30

[2] CRYSTAL-STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS [J].

BROWN, T ;

LEONARD, GA ;

BOOTH, ED ;

CHAMBERS, J .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 207 (02) :455-457

[3]

BROWN T, 1986, P NATL ACAD SCI USA, V83, P2401

[4] G(SYN).A(ANTI) MISMATCH FORMATION IN DNA DODECAMERS AT ACIDIC PH - PH-DEPENDENT CONFORMATIONAL TRANSITION OF G.A MISPAIRS DETECTED BY PROTON NMR [J].

GAO, XL ;

PATEL, DJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (15) :5178-5182

[5] STABILITIES OF NEAREST-NEIGHBOR DOUBLETS IN DOUBLE-HELICAL DNA DETERMINED BY FITTING CALCULATED MELTING PROFILES TO OBSERVED PROFILES [J].

GOTOH, O ;

TAGASHIRA, Y .

BIOPOLYMERS, 1981, 20 (05) :1033-1042

[6] STRUCTURE OF AN ADENINE.CYTOSINE BASE PAIR IN DNA AND ITS IMPLICATIONS FOR MISMATCH REPAIR [J].

HUNTER, WN ;

BROWN, T ;

ANAND, NN ;

KENNARD, O .

NATURE, 1986, 320 (6062) :552-555

[7] STRUCTURAL FEATURES AND HYDRATION OF D(C-G-C-G-A-A-T-T-A-G-C-G) - A DOUBLE HELIX CONTAINING 2 GA MISPAIRS [J].

HUNTER, WN ;

BROWN, T ;

KENNARD, O .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1986, 4 (02) :173-&

[8] DETECTION OF A GUANINE.ADENINE BASE PAIR IN A DECADEOXYRIBONUCLEOTIDE BY PROTON MAGNETIC-RESONANCE SPECTROSCOPY [J].

KAN, LS ;

CHANDRASEGARAN, S ;

PULFORD, SM ;

MILLER, PS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (14) :4263-4265

[9]

Kennard O., 1987, NUCL ACIDS MOL BIOL, V1, P25

[10] BASE-PAIRING INVOLVING DEOXYINOSINE - IMPLICATIONS FOR PROBE DESIGN [J].

MARTIN, FH ;

CASTRO, MM ;

ABOULELA, F ;

TINOCO, I .

NUCLEIC ACIDS RESEARCH, 1985, 13 (24) :8927-8938

← 1 2 →