CLONING AND ANALYSIS OF THE 5' FLANKING SEQUENCE OF THE RAT N-METHYL-D-ASPARTATE RECEPTOR-1 (NMDAR1) GENE

被引：49

作者：

BAI, G

KUSIAK, JW

机构：

[1] National Institute on Aging, National Institutes of Health, Molecular Neurobiology Unit, Baltimore, MD 21224

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1152卷 / 01期

关键词：

N-METHYL-D-ASPARTATE RECEPTER-1 GENE; TRANSCRIPTION START SITE; PROMOTER; HOUSEKEEPING GENE;

D O I：

10.1016/0005-2736(93)90249-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned and analyzed a 3.8 kb EcoRI fragment of the rat NMDAR1 gene. It contains 3 kb of promoter/enhancer region, exon 1 and a portion of intron 1. Two major transcription start sites were identified at - 276 and - 238 from the first nucleotide in codon 1. One GSG and two SP1 motifs, but no TATA/CAAT boxes, exist in the region proximal to the transcription start sites. Our results suggest that NMDAR1 has the characteristics of a housekeeping gene and may be regulated by immediate-early genes.

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页码：197 / 200

页数：4

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