DUAL MICROCOLUMN IMMUNOAFFINITY LIQUID-CHROMATOGRAPHY - AN ANALYTICAL APPLICATION TO HUMAN PLASMA-PROTEINS

被引：19

作者：

FLURER, CL ^{[1
]}

NOVOTNY, M ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 06期

关键词：

D O I：

10.1021/ac00054a028

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Dual-column microcolumn immunoaffinity chromatography was accomplished by coupling immunoaffinity (IAC) and reversed-phase high-performance liquid chromatography (RP-HPLC) analysis columns. The IAC support was prepared by hydrophobically adsorbing the appropriate antibody onto an octyl silica-based stationary phase contained in a 250-mum-i.d. fused-silica microcolumn. Once the antigen was captured, the antigen-subtracted human plasma could be sent to the RP-HPLC microcolumn for plasma profiling. The antigen was quantitated by desorbing it from the IAC column and transferring it to the RP-HPLC system. Human plasma profiles were generated after removal of albumin, and transferrin and alpha1-antitrypsin were quantitated with nanogram sensitivity. All analyses were accomplished with less than 1 muL of human plasma.

引用

页码：817 / 821

页数：5

共 29 条

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[3]

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