SIMPLE METHOD FOR THE SIMULTANEOUS DETERMINATION OF NORADRENALINE, DOPAMINE AND SEROTONIN BY STEPWISE ELUTION FROM A SHORT COLUMN OF WEAK CATION-EXCHANGE RESIN

被引：33

作者：

OGASAHARA, S

MANDAI, T

YAMATODANI, A

WATANABE, T

WADA, H

SEKI, T

机构：

[1] Department of Pharmacology II and Genetics, Osaka University School of Medicine, Kita-ku, Osaka, 530, 3-57

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 180卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)80180-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple method for the simultaneous determination of noradrenaline, dopamine and serotonin using a short column of Amberlite CG-50 is described. Noradrenaline and dopamine were eluted from the column with phosphate buffers containing 1.5 and 4.0% boric acid, respectively, and then serotonin was eluted with 1.0 N HCl. Catecholamines were determined by a modification of the ethylenediamine condensation method using potassium ferricyanide as oxidant and isobutanol for extraction of the fluorophores. Serotonin was measured by the acidic o-phthalaldehyde method. The method was applied to the simultaneous determination of noradrenaline, dopamine and serotonin in discrete regions of rat brain. © 1979.

引用

页码：119 / 126

页数：8

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