ESTABLISHMENT OF 2 NEW CELL-LINES DERIVED FROM HUMAN BREAST CARCINOMAS WITH HER-2/NEU AMPLIFICATION

被引:54
作者
MELTZER, P
LEIBOVITZ, A
DALTON, W
VILLAR, H
KUTE, T
DAVIS, J
NAGLE, R
TRENT, J
机构
[1] UNIV MICHIGAN, CTR CANC, DEPT RADIAT ONCOL, ANN ARBOR, MI 48109 USA
[2] UNIV ARIZONA, COLL MED, DEPT INTERNAL MED, TUCSON, AZ 85724 USA
[3] UNIV ARIZONA, COLL MED, DEPT SURG, TUCSON, AZ 85724 USA
[4] UNIV ARIZONA, COLL MED, ARIZONA CANC CTR, DEPT PATHOL, TUCSON, AZ 85724 USA
[5] WAKE FOREST UNIV, BOWMAN GRAY SCH MED, HEMATOL ONCOL SECT, WINSTON SALEM, NC 27103 USA
关键词
D O I
10.1038/bjc.1991.164
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Two human cell lines (UACC-812 and 893), both containing significant amplification of the HER-2/neu gene, were established from biopsy specimens of breast carcinomas. One patient had Stage II breast carcinoma; the other had metastatic disease. Characterisation of these lines has revealed that both are highly aneuploid containing multiple clonal chromosome alterations, have doubling times near 100 h, and are oestrogen and progesterone receptor negative. Electron microscopy demonstrates that both lines contain numerous microvilli, cytoplasmic filaments, multivesicular bodies, and demosomes. Immunoblot analysis for P-glycoprotein using the monoclonal antibody C219 was negative for both patient cell lines. These relatively rare cell lines may represent a useful model to investigate human breast carcinomas.
引用
收藏
页码:727 / 735
页数:9
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