PHYSICAL-CHEMICAL CHARACTERIZATION AND CARBOHYDRATE-BINDING ACTIVITY OF THE A-SUBUNIT AND B-SUBUNIT OF THE BANDEIRAEA-SIMPLICIFOLIA-I ISOLECTINS

被引:52
作者
MURPHY, LA [1 ]
GOLDSTEIN, IJ [1 ]
机构
[1] UNIV MICHIGAN,DEPT BIOL CHEM,ANN ARBOR,MI 48109
关键词
D O I
10.1021/bi00589a030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bandeiraea simplicifolia I plant seed isolectins comprise a family of tetrameric α-d-galactopyranosyl-binding glycoproteins composed of various combinations of two different kinds of subunits designated A and B. Subtypes of the A (Aa, Ab, Ac, Ad, and Ae) and B (Ba, Bb, Bc, Bd, and Be) subunits were demonstrated by isoelectric focusing in 8 M urea. Although the content of subunit subtypes varies from seed to seed (e.g., some seeds contain only B subunits, others only A subunits), subtypes Ac and Bc predominate in a natural mixture of the isolectins. Two-dimensional agar gel diffusion studies indicate that, in addition to common structural features, each subunit contains its own distinct antigenic determinants. Although the A and B subunits have closely similar amino acid compositions, they differ markedly in one respect: the B subunit has one methionine residue whereas the A subunit contains no methionine. The neutral carbohydrate content of both subunits is identical. The ability of biopolymers and synthetic glycoproteins to precipitate A4 and B4, as well as the capacity of sugars and oligosaccharides to inhibit precipitate formation, was examined. On the basis of these studies, it is suggested that hydrogen bonding occurs between the hydrogen atoms of the C-3 and C-4 hydroxyl groups of α-d-GalNAcp and α-d-Galp units and the A and B subunits, respectively. © 1979, American Chemical Society. All rights reserved.
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页码:4999 / 5005
页数:7
相关论文
共 24 条
[1]   2-DIMENSIONAL GEL-ELECTROPHORESIS OF MEMBRANE PROTEINS [J].
AMES, GFL ;
NIKAIDO, K .
BIOCHEMISTRY, 1976, 15 (03) :616-623
[2]   DISC ELECTROPHORESIS [J].
BREWER, JM ;
ASHWORTH, RB .
JOURNAL OF CHEMICAL EDUCATION, 1969, 46 (01) :41-&
[3]  
CARTER WG, 1975, J BIOL CHEM, V250, P2756
[4]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[5]   EQUILIBRIUM DIALYSIS AND CARBOHYDRATE-BINDING STUDIES ON 2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSYL-BINDING LECTIN FROM BANDEIRAEA-SIMPLICIFOLIA SEEDS [J].
EBISU, S ;
IYER, PNS ;
GOLDSTEIN, IJ .
CARBOHYDRATE RESEARCH, 1978, 61 (MAR) :129-138
[6]   SPECTROSCOPIC DETERMINATION OF TRYPTOPHAN AND TYROSINE IN PROTEINS [J].
EDELHOCH, H .
BIOCHEMISTRY, 1967, 6 (07) :1948-&
[7]   METHODS FOR INVESTIGATING THE ESSENTIAL GROUPS FOR ENZYME ACTIVITY [J].
FRAENKELCONRAT, H .
METHODS IN ENZYMOLOGY, 1957, 4 :247-269
[8]  
Habeeb A F, 1972, Methods Enzymol, V25, P457, DOI 10.1016/S0076-6879(72)25041-8
[9]  
HAYES CE, 1974, J BIOL CHEM, V249, P1904
[10]   QUANTITATIVE STUDIES ON INTERACTION OF CONCANAVALIN-A, CARBOHYDRATE-BINDING PROTEIN OF JACK BEAN, WITH MODEL CARBOHYDRATE-PROTEIN CONJUGATES [J].
IYER, RN ;
GOLDSTEIN, IJ .
IMMUNOCHEMISTRY, 1973, 10 (05) :313-322