IDENTIFICATION OF ENTERICALLY TRANSMITTED HEPATITIS-VIRUS PARTICLES BY SOLID-PHASE IMMUNE ELECTRON-MICROSCOPY

被引：15

作者：

HUMPHREY, CD ^{[1
]}

COOK, EH ^{[1
]}

BRADLEY, DW ^{[1
]}

机构：

[1] CTR DIS CONTROL, WHO COLLABORAT CTR RES & REFERENCE VIRAL HEPATITIS, ATLANTA, GA 30333 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1990年 / 29卷 / 02期

关键词：

Hepatitis; Immune electron microscopy; Solid-phase immune electron microscopy; Virus identification;

D O I：

10.1016/0166-0934(90)90111-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Small 'featureless' viruses (< 50 nm) are difficult to identify by routine immune electron microscopy techniques, particularly when they are mixed with debris from stool or cell culture extracts. A combination of conventional immune electron microscopy (IBM) and solid phase IBM (SPIBM) methodologies was used to identify hepatitis A virus (HAV) in stool and cell culture extracts and non-A non-B hepatitis (hepatitis B) in stool extracts. Compared with conventional IBM, the modified SPIBM method resulted in a significant increase in the number of particles observed. Several small aggregates, each containing 2-20 particles, were observed scattered randomly within most grid squares. Similar results were seen with stool extracts from hepatitis B (HBV) infections. The SPIBM method is a simple, highly sensitive specific assay that facilitates rapid identification of enteric hepatitis viruses. Several experiments were done to characterize the effects of altered physical environment within the assay and to evaluate potential modifications. © 1990.

引用

页码：177 / 188

页数：12

共 17 条

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