THE TRANSCRIPTION FACTOR LF-A1 INTERACTS WITH A BIPARTITE RECOGNITION SEQUENCE IN THE PROMOTER REGIONS OF SEVERAL LIVER-SPECIFIC GENES

被引：70

作者：

RAMJI, DP ^{[1
]}

TADROS, MH ^{[1
]}

HARDON, EM ^{[1
]}

CORTESE, R ^{[1
]}

机构：

[1] INST BIOL 2,W-7880 FREIBURG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 05期

关键词：

D O I：

10.1093/nar/19.5.1139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription factors LF-A1 and LF-B1 are required for the cell-specific expression of the human alpha-1-antitrypsin gene in hepatocytes. We report here the purification and preliminary characterization of LF-A1. This protein, purified to homogeneity from calf liver nuclei by site-specific DNA affinity chromatography and reverse-phase HPLC, has a molecular mass of 40 kDa. Binding sites of LF-A1 are present in the promoter regions of several genes expressed in the liver (alpha-1-antitrypsin, apolipoproteins A1, B1, A4 and pyruvate kinase). Interestingly, the binding site of LF-A1 is bipartite and consists of two short sequence motifs (consensus: TGGAC(T/CT/C) and TGGCCC) separated by a variable 'spacer' region. Insertion or deletion of 1-4 nucleotides in the 'spacer' region of the site in the alpha-1-antitrypsin promoter does not abolish DNA binding.

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页码：1139 / 1146

页数：8

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