ISOLATION OF SIMPLE-SEQUENCE LOCI FOR USE IN POLYMERASE CHAIN REACTION-BASED DNA FINGERPRINTING

被引：168

作者：

RASSMANN, K ^{[1
]}

SCHLOTTERER, C ^{[1
]}

TAUTZ, D ^{[1
]}

机构：

[1] UNIV MUNICH,INST GENET & MIKROBIOL,MARIA WARDSTR 1A,W-8000 MUNICH 2,GERMANY

来源：

ELECTROPHORESIS | 1991年 / 12卷 / 2-3期

关键词：

D O I：

10.1002/elps.1150120205

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simple sequences are short regions of tandem repetitions of mono-, di-, tri-, or tetranucleotide motifs and occur as repetitive elements in all eukaryotic genomes. These regions tend to be hypervariable in length and can therefore be exploited for DNA fingerprinting purposes, using the polymerase chain reaction with primers flanking such regions. We describe how suitable simple-sequence loci can be isolated from any given eukaryotic DNA. We show the DNA sequences for a number of variants of such loci and discuss the current results on their usefulness for DNA fingerprinting.

引用

页码：113 / 118

页数：6

共 12 条

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