POLYMERASE CHAIN-REACTION AMPLIFICATION FROM PLASMODIUM-FALCIPARUM ON DRIED BLOOD SPOTS

被引：27

作者：

LONG, GW

FRIES, L

WATT, GH

HOFFMAN, SL

机构：

[1] JOHNS HOPKINS UNIV, SCH HYG & PUBL HLTH, CTR VACCINE RES, BALTIMORE, MD 21205 USA

[2] ARMED FORCES RES INST MED SCI, DEPT MED, BANGKOK 10400, THAILAND

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1995年 / 52卷 / 04期

关键词：

D O I：

10.4269/ajtmh.1995.52.344

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We report a simple method for the polymerase chain reaction (PCR) amplification of whole blood samples collected on filter paper. The blood spot was used directly in the PCR after treatment with methanol. We evaluated this assay using clinical samples collected from subjects in a Plasmodium falciparum vaccine trial and from samples collected during a hospital-based study in Thailand. Specimens prepared from heparinized blood samples were successfully amplified following pretreatment with heparinase. Sensitivity was 100% when compared with thick blood film results in the vaccine trial (range = 4-60 parasites/mu l, median = 8/mu l) and 94.6% (range = 3-133,988 parasites/mu l, median = 616/mu l) in the hospital study.

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收藏

页码：344 / 346

页数：3

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