MEASUREMENT OF DESMOSINE AND ISODESMOSINE BY CAPILLARY ZONE ELECTROPHORESIS

被引：21

作者：

GIUMMELLY, P

BOTTON, B

FRIOT, R

PRIMAPUTRA, D

ATKINSON, J

机构：

[1] UNIV NANCY 1,FAC PHARM,PHARMACOL CARDIOVASC LAB,F-54001 NANCY,FRANCE

[2] UNIV NANCY 1,FAC SCI,INRA,BIOL FORESTIERE LAB,F-54506 VANDOEUVRE NANCY,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 710卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00487-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Desmosine (DES) and isodesmosine (IDE) were separated and quantitated by a simple and sensitive capillary zone electrophoretic (CZE) method, using hydrostatic injection and direct UV detection at 254 or 185 nm. Two different electrophoretic mobilities for the two isoforms were observed in 90 mM phosphoric acid pH 2.2. The presence of a mixture of amino acids in the sample did not affect the separation of DES and IDE. The method was successfully used to quantitate the amounts of DES and IDE in elastin hydrolysates.

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页码：357 / 360

页数：4

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