ONE-STEP ELECTROBLOTTING OF 2- TO 100-KDA PROTEINS ONTO A PVDF MEMBRANE

被引：2

作者：

PARRADO, J ^{[1
]}

AYALA, A ^{[1
]}

MACHADO, A ^{[1
]}

机构：

[1] UNIV SEVILLA,FAC FARM,DEPT BIOQUIM BROMATOL & TOXICOL,E-41012 SEVILLE,SPAIN

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 53卷 / 03期

关键词：

ELECTROBLOTTING; PROTEINS; PEPTIDES; PVDF;

D O I：

10.1007/BF02783502

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article describes a method for electroblotting peptides and small proteins (<100 kDa) from tricine gels onto a PVDF membrane. The major potential problem with these types of procedures is that proteins tend to stay in the gel under conditions where peptides are effectively eluted. The suggested protocol allows the complete transfer and binding of proteins and peptides in the range of 2-97 kDa.

引用

页码：285 / 292

页数：8

共 12 条

[1] Gershoni J.M., Palade G.M., Anal. Biochem., 131, pp. 1-15, (1983)
[2] Towbin H., Gordon J., J. Immunol. Methods, 72, pp. 313-340, (1984)
[3] Gershoni J.M., Trends Biochem. Sci., 10, pp. 103-106, (1985)
[4] Bers G., Garfin D., BioTechniques, 3, pp. 276-288, (1985)
[5] Matsudaira P., J. Biol. Chem., 262, (1987)
[6] Lin W., Kasamatsu H., Anal. Biochem., 128, pp. 302-311, (1983)
[7] Burnete W.N., Anal. Biochem., 112, pp. 195-203, (1981)
[8] Beck S., Anal. Biochem., 170, pp. 361-366, (1988)
[9] Karey K.P., Sirbasku D.A., Anal. Biochem., 178, pp. 255-259, (1989)
[10] Schagger H., Von Jagow G., Anal. Biochem., 166, pp. 368-379, (1987)

← 1 2 →