UPTAKE, METABOLISM, AND ELIMINATION OF THE LAMPRICIDE 3-TRIFLUOROMETHLY-4-NITROPHENOL BY LARGEMOUTH BASS (MICROPTERUS-SALMOIDES)

Largemouth bass (Micropterus salmoides) exposed to a 1.0-µg/mL solution of the lampricide 3-trifluoromethyl-4-nitro[14C]phenol (TFM) for up to 24 h accumulated radioactive residues in all tissues analyzed at each of five successive sampling periods. Maximum concentrations occurred after 8 h in brain and muscle and after 12h in blood, liver, kidney, and head plus viscera. Concentrations of radioactivity in the bile increased throughout the experiment. In a second group of fish exposed to 1.0 µg/mL of [14C]TFM for 12 h and then transferred to lampricide-free flowing water, the concentration of radioactive materials in tissues generally decreased with time throughout a 72-h elimination period. No TFM was detected in muscle tissue 12 h after the fish were transferred to lampricide-free water. The presence of conjugated TFM in the bile was confirmed. Hexane/ether extracts contained [14C]TFM and other unidentified 14C materials from muscle and head plus viscera, whereas methanol extracts taken after the hexane/ether extraction contained only a negligible amount of [14C]TFM but large quantities of unidentified, polar 14C compounds. © 1979, American Chemical Society. All rights reserved.