RAPID METHOD FOR PURIFYING BACTERIAL DEOXYRIBONUCLEIC-ACID

被引：21

作者：

GIBSON, DM

OGDEN, ID

机构：

[1] Ministry of Agriculture, Torry Research Station, Aberdeen, AB9 8DG

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1979年 / 46卷 / 03期

关键词：

D O I：

10.1111/j.1365-2672.1979.tb00839.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA was purified from bacterial cell lysates by treatment with RNA ase and Pronase followed by chromatography on a column of Sepharose 4B with standard saline citrate buffer as eluant. It has the same base composition as DNA prepared by‐Marmur's (1961) method. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：421 / 423

页数：3

共 8 条

[1] Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid [J].