LIPOPROTEINS SECRETED BY CULTURED RAT HEPATOCYTES CONTAIN THE ANTIOXIDANT 1-ALK-1-ENYL-2-ACYLGLYCEROPHOSPHOETHANOLAMINE

Monolayer cultures of rat hepatocytes have been examined for their ability to secrete ethanolamine plasmalogen as a component of nascent lipoproteins. In culture medium from these cells, ethanolamine plasmalogen comprises approx. 20-30% of total ethanolamine glycerophospholipids when measured either as phospholipid mass or by the incorporation of [1-3H]ethanolamine. An approximately equal distribution of the plasmalogen was found throughout all lipoprotein density fractions. The content of plasmalogen in whole rat serum, was 36% of total ethanolamine glycerophospholipids. In contrast, in rat liver and cultured hepatocytes the amount of ethanolamine plasmalogen was 5-fold lower than in serum or culture medium (approx. 5% of total ethanolamine phospholipids). Normal human plasma also contains ethanolamine plasmalogen in relatively large amounts (approx. 50% of total ethanolamine phospholipids). Thus, a major function of plasmalogen biosynthetic enzymes in liver may be the provision of ethanolamine plasmalogen for secretion into lipoproteins. Previous studies (e.g., Zoeller, R.A.. (1988) J. Biol. Chem. 263, 11590-11596) have suggested that ethanolamine plasmalogen may function as an antioxidant for the protection of lipid and protein membrane components against oxidation. Oxidized, but not native, low-density lipoprotein is rapidly taken up by macrophages with the formation of foam cells characteristic of atherosclerotic lesions (Steinbrecher, U.P.. (1984) Proc. Natl. Acad. Sci. USA 81, 3883-3887). Thus, the presence of plasmalogen as part of newly secreted lipoprotein particles may prevent their oxidation and subsequent uptake by macrophages. © 1990.