Improved preservation of glycogen in unfixed cyanobacteria embedded at-82 degrees C in nanoplast

被引：4

作者：

Froesch, Dieter ^{[1
]}

Westphal, Christel ^{[1
]}

Boehme, Herbert ^{[2
]}

机构：

[1] Univ Ulm, Sect EM, D-7900 Ulm, Germany

[2] Univ Konstanz, Fac Biol, D-7750 Constance, Germany

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1987年 / 35卷 / 01期

关键词：

aminoplast; cryo-embedding; cryo-substitution; cyanobacteria; glycogen; melamine resin; Nanoplast; polysaccharides; thin sectioning; unfixed cells;

D O I：

10.1177/35.1.3098832

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A technique is presented for embedding unfixed cells at -82 degrees C in Nanoplast, a water-soluble melamine resin. By use of this procedure, improved ultrastructural preservation is demonstrated in cyanobacteria. Sections treated in this way are suitable for histochemical techniques such as Thiery's silver staining method for glycogen.

引用

页码：119 / 121

页数：3

共 8 条

[1] PHOTOCHEMICAL ACTIVITY AND COMPONENTS OF MEMBRANE PREPARATIONS FROM BLUE-GREEN-ALGAE .1. COEXISTENCE OF 2 PHOTOSYSTEMS IN RELATION TO CHLOROPHYLL ALPHA AND REMOVAL OF PHYCOCYANIN [J].