STUDIES ON THE MORPHOGENETIC RESPONSE OF MAIZE TISSUE-CULTURES OF DIFFERENT ORIGIN

The participation of the genotype and of organ specifity effect in the quality of morphogenetic response (callogenesis, bud and root formation) of primary maize explants has been investigated. The presence of synthetic auxins - especially 2,4-D at 1 to 5 mg 1-1 conc. - in cultivation medium was essential for both callus formation and continuous growth of tissue and suspension cultures. Anatomic structure of callus cultures is permanently heterogeneous, their growth is ensured by the action of meristems of the type found in root tips, and by repeated callogenesis from malformed roots. Adventive buds and plants could be regenerated only from cultures of embryonal origin (of one line). The presence or absence of the endosperm gene opaque" did not influence callogenesis intensity in cultures of isolated embryos; however the morphogenetic response was clearly "line specific". © 1979 Academia."