TRANSFER OF CYTOPLASMIC MALE-STERILITY BY SPONTANEOUS ANDROGENESIS IN TOBACCO (NICOTIANA-TABACUM-L)

被引：7

作者：

HORLOW, C ^{[1
]}

DEFRANCE, MC ^{[1
]}

POLLIEN, JM ^{[1
]}

GOUJAUD, J ^{[1
]}

DELON, R ^{[1
]}

PELLETIER, G ^{[1
]}

机构：

[1] INST RECH TABAC SEITA, F-24100 BERGERAC, FRANCE

来源：

EUPHYTICA | 1993年 / 66卷 / 1-2期

关键词：

CYTOPLASMIC MALE STERILITY; NICOTIANA-TABACUM; SPONTANEOUS ANDROGENESIS; RAC-; MUTANT; CHROMOSOME DOUBLING;

D O I：

10.1007/BF00023507

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Five cytoplasmic male sterile lines of Nicotiana tabacum homozygous for a dominant rootless mutation [Rac-] were used as female parents in crosses with three male fertile varieties. Androgenetic haploids were selected by their ability to form plantlets with a normal root system, whereas hybrid plantlets failed to grow. High frequencies of androgenetic haploid plants were obtained with N. debneyi cytoplasm, and with one paternal genotype. Chromosome doubling was performed using three different methods (pollination, in vitro culture, acenaphthen) and cytoplasmic male sterile doubled haploid plants have been obtained. The results demonstrate the efficiency of this method for a rapid cytoplasm transfer in Nicotiana tabacum; the integrity of the cytoplasm is conserved. For the first time, we possess a counter selectable marker universally usable for Nicotiana tabacum and closely related species.

引用

页码：45 / 53

页数：9

共 37 条

[1] MORPHOLOGICAL-CHARACTERISTICS AND CHLOROPLAST DNA DISTRIBUTION IN DIFFERENT CYTOPLASMIC PARASEXUAL HYBRIDS OF NICOTIANA-TABACUM [J].