SENDAI VIRUS-INDUCED CELL-FUSION

This chapter discusses the mechanism and details of cell fusion by hemagglutinating virus of japan (HVJ). Sendai virus (HVJ) can fuse cells with viral receptors on their surface, including almost all cultured cells derived from mammals and fowls. in general, cells of established lines and transformed cells are fused more readily than differentiated cells, ∼ and HVJ can fuse both cells in suspension and plated cells in culture dishes. The use of cells in suspension is easier for quantitative estimation of cell fusion efficiency. The chapter describes the mechanism of fusion of Ehdich ascites tumor (EAT) cells induced by HVJ. This chapter discusses that Ehdich ascites tumor cells are rapidly fused by HVJ at 37 °, under conditions supplying calcium ions and allowing energy generation. The initial reaction is disruption of the plasma membranes, induced by the function of the fusogenic N-terminal domain of F1. © 1993, Elsevier Inc. All rights reserved.