PATTERNING OF THE NEOCORTICAL PROJECTIONS FROM THE RAPHE NUCLEI IN PERINATAL RATS - INVESTIGATION OF POTENTIAL ORGANIZATIONAL MECHANISMS

被引:24
作者
BENNETTCLARKE, CA
HANKIN, MH
LESLIE, MJ
CHIAIA, NL
RHOADES, RW
机构
[1] Department of Anatomy, Medical College of Ohio, Toledo, Ohio
关键词
SEROTONIN; THALAMOCORTICAL AFFERENTS; SOMATOSENSORY; DEVELOPMENT; TISSUE TRANSPLANTATION;
D O I
10.1002/cne.903480209
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Serotoninergic (5-HT) fibers in the cerebral cortex of perinatal rats have a pattern that coincides with the boundaries of primary sensory areas and within the primary somatosensory cortex form the rattunculus. This patterned immunoreactivity (IR) appears about 60 hours after birth and disappears between postnatal days (P-) 12 and 15. Three experiments were carried out to evaluate mechanisms that might underlie the precise patterning of the 5-HT-IR. Retrograde labelling with fluorescent tracers in perinatal rats revealed only a coarse rostrocaudal topography in the raphe-cortical projection and the existence of raphe cells projecting to multiple cortical locations. Thus, a precise point-to-point, raphe-cortical projection does not underlie the patterned cortical 5-HT-IR. Ablation of the thalamus prior to the age at which patterned 5-HT-IR could be seen in the developing cortex caused a complete loss of patterned immunoreactivity. This suggests that 5-HT fibers may require the presence of thalamocortical axons to achieve the pattern observed in normal animals. Serotoninergic raphe neurons transplanted to the cortices of newborn rats exhibited extensive axonal outgrowth, but did not form a somatotopic pattern. This result also suggests that specific spatiotemporal interactions between growing 5-HT and thalamocortical axons may be necessary for the somatotopic patterning of the former fibers. (C) 1994 Wiley-Liss, Inc.
引用
收藏
页码:277 / 290
页数:14
相关论文
共 25 条
[1]   THE SOURCE OF THE TRANSIENT SEROTONINERGIC INPUT TO THE DEVELOPING VISUAL AND SOMATOSENSORY CORTICES IN RAT [J].
BENNETTCLARKE, CA ;
CHIAIA, NL ;
CRISSMAN, RS ;
RHOADES, RW .
NEUROSCIENCE, 1991, 43 (01) :163-183
[2]   SEROTONIN-1B RECEPTORS IN THE DEVELOPING SOMATOSENSORY AND VISUAL CORTICES ARE LOCATED ON THALAMOCORTICAL AXONS [J].
BENNETTCLARKE, CA ;
LESLIE, MJ ;
CHIAIA, NL ;
RHOADES, RW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (01) :153-157
[3]   CORRESPONDENCE BETWEEN 5-HT2 RECEPTORS AND SEROTONERGIC AXONS IN RAT NEOCORTEX [J].
BLUE, ME ;
YAGALOFF, KA ;
MAMOUNAS, LA ;
HARTIG, PR ;
MOLLIVER, ME .
BRAIN RESEARCH, 1988, 453 (1-2) :315-328
[4]   CONNECTIONS OF MEDIAN AND DORSAL RAPHE NUCLEI IN RAT - AUTORADIOGRAPHIC AND DEGENERATION STUDY [J].
CONRAD, LCA ;
LEONARD, CM ;
PFAFF, DW .
JOURNAL OF COMPARATIVE NEUROLOGY, 1974, 156 (02) :179-205
[5]   ONTOGENY OF THE SEROTONERGIC PROJECTION TO RAT NEOCORTEX - TRANSIENT EXPRESSION OF A DENSE INNERVATION TO PRIMARY SENSORY AREAS [J].
DAMATO, RJ ;
BLUE, ME ;
LARGENT, BL ;
LYNCH, DR ;
LEDBETTER, DJ ;
MOLLIVER, ME ;
SNYDER, SH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) :4322-4326
[6]   ULTRASTRUCTURAL FEATURES OF SEROTONIN NEURONS GRAFTED TO ADULT-RAT HIPPOCAMPUS - AN IMMUNOCYTOCHEMICAL ANALYSIS OF THEIR CELL-BODIES AND AXON TERMINALS [J].
DASZUTA, A ;
CHAZAL, G ;
GARCIA, S ;
OLESKEVICH, S ;
DESCARRIES, L .
NEUROSCIENCE, 1991, 42 (03) :793-811
[7]   THE SEROTONIN NEURONS IN NUCLEUS RAPHE DORSALIS OF ADULT-RAT - A LIGHT AND ELECTRON-MICROSCOPE AUTORADIOGRAPHIC STUDY [J].
DESCARRIES, L ;
WATKINS, KC ;
GARCIA, S ;
BEAUDET, A .
JOURNAL OF COMPARATIVE NEUROLOGY, 1982, 207 (03) :239-254
[8]   SEROTONIN NERVE-TERMINALS IN ADULT RAT NEOCORTEX [J].
DESCARRIES, L ;
BEAUDET, A ;
WATKINS, KC .
BRAIN RESEARCH, 1975, 100 (03) :563-588
[9]  
ERZURUMLU RS, 1990, DEV BRAIN RES, V56, P229
[10]   STRUCTURAL AND FUNCTIONAL-ANALYSIS OF RAPHE NEURON IMPLANTS INTO DENERVATED RAT SPINAL-CORD [J].
FOSTER, GA ;
ROBERTS, MHT ;
WILKINSON, LS ;
BJORKLUND, A ;
GAGE, FH ;
HOKFELT, T ;
SCHULTZBERG, M ;
SHARP, T .
BRAIN RESEARCH BULLETIN, 1989, 22 (01) :131-137