AGROBACTERIUM-MEDIATED TRANSFORMATION OF SWEET ORANGE AND REGENERATION OF TRANSGENIC PLANTS

被引：116

作者：

PENA, L

CERVERA, M

JUAREZ, J

NAVARRO, A

PINA, JA

DURANVILA, N

NAVARRO, L

机构：

[1] Dpto. de protección vegetal y biotecnología, Institute Valenciano de Investigaciones Agrarias, Moncada, E-46113, Apartado oficial

来源：

PLANT CELL REPORTS | 1995年 / 14卷 / 10期

关键词：

CITRUS SINENSIS L OSBECK CV PINEAPPLE; SWEET ORANGE; AGROBACTERIUM TUMEFACIENS; GENETIC TRANSFORMATION;

D O I：

10.1007/BF00232724

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and beta-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.

引用

页码：616 / 619

页数：4

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