HEAT-SHOCK PROTEINS IN ACUTE OTITIS-MEDIA

被引：5

作者：

EGUSA, K ^{[1
]}

HUANG, CC ^{[1
]}

HADDAD, J ^{[1
]}

机构：

[1] COLUMBIA PRESBYTERIAN MED CTR,BABIES HOSP,MED CTR,DEPT OTOLARYNGOL HEAD & NECK SURG,NEW YORK,NY 10032

来源：

LARYNGOSCOPE | 1995年 / 105卷 / 07期

关键词：

D O I：

10.1288/00005537-199507000-00007

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Heat shock proteins (hsps) are essential for the survival of cells under an environmental insult. To elucidate the relationship between these intracellular proteins and acute otitis media (AOM), the authors studied hsp production in guinea pigs with AOM induced by inoculation of Streptococcus pneumoniae into the middle ear. Animals were sacrificed 1, 3, or 5 days after inoculation. Immunocytochemical study showed localization of hsp70 within the epithelium of infected ears, while the normal control epithelium showed very light staining. Western blot tests of the mucosal extraction confirmed the presence of hsp70 in otitis media. Enzyme-linked immunosorbent assay (ELISA) showed that hsp levels were higher in the infected mucosa than in the normal control mucosa. In contrast, hsp60 was not stimulated in infected ears. This study showed that hsp70, and not hsp60, was produced in the AOM model.

引用

页码：708 / 713

页数：6

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