ALPHA-D-GALACTOSIDASE (EC-3.2.1.22) FROM BIFIDOBACTERIUM-LONGUM

被引：23

作者：

GARRO, MS ^{[1
]}

DEGIORI, GS ^{[1
]}

DEVALDEZ, GF ^{[1
]}

OLIVER, G ^{[1
]}

机构：

[1] CERELA,RA-4000 TUCUMAN,ARGENTINA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00892.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

alpha-Galactosidase activity in Bifidobacterium longum was maximal in a range of 40-45 degrees C and at pH of 5.8. At temperatures above 60 degrees C the enzyme was completely inactivated, but it maintained a 100% activity during storage at low temperatures (4, -30, -70 degrees C) for 3 months. The addition of Hg2+ to the reaction buffer produced a strong inhibitory effect while Mn2+ exerted a slight positive effect upon activity. The addition of EDTA, inhibitors and other metal ions had no effect on the enzyme alpha-galactosidase.

引用

页码：16 / 19

页数：4

共 12 条

[1]

Church FC, 1980, DEV IND MICROBIOL, V21, P339

[2]

Dawson R.M.C., 1986, DATA BIOCH RES, P427

[3]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[4] CHARACTERIZATION OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS-FERMENTUM [J].