ORIGIN OF YOLK-DNA IN XENOPUS-LAEVIS

被引:10
作者
OPRESKO, L [1 ]
WILEY, HS [1 ]
WALLACE, RA [1 ]
机构
[1] OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37830
来源
JOURNAL OF EXPERIMENTAL ZOOLOGY | 1979年 / 209卷 / 03期
关键词
D O I
10.1002/jez.1402090303
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Xenopus laevis serum and plasma was found to contain an average of 25 μg DNA/ml. Isolated X. laevis oocytes incubated in medium containing 25 μg DNA/ml labeled with either 125I, 32P or 14C and from three different sources (bovine, E. coli and X. laevis), incorporated the label at an average rate of 0.11 ng·mm−·hr−1. Sucrose gradient fractionation of oocytes revealed that 40–75% of the acid‐precipitable label incorporated was associated with the yolk platelets. Additional incubations of oocytes in unlabeled medium demonstrated that the DNA incorporated into the yolk platelets was undergoing turnover; only 20% of the yolk‐associated DNA was still present after a one‐week incubation. Our data suggest that yolk‐DNA arises by the adventitious uptake of DNA present in the maternal serum by vitellogenic oocytes. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company
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页码:367 / 376
页数:10
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