OVARIAN GRANULOSA CELL-DERIVED INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS - MODULATORY ROLE OF FOLLICLE-STIMULATING-HORMONE

被引:79
作者
ADASHI, EY
RESNICK, CE
HURWITZ, A
RICCIARELLI, E
HERNANDEZ, ER
ROSENFELD, RG
机构
[1] UNIV MARYLAND,SCH MED,DEPT PHYSIOL,BALTIMORE,MD 21201
[2] STANFORD UNIV,MED CTR,DEPT PEDIAT,STANFORD,CA 94143
关键词
D O I
10.1210/endo-128-2-754
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Recent observations disclosed the multiplicity of granulosa cell-derived high affinity insulin-like growth factor binding proteins (IGFBPs) and revealed the striking ability of high doses of FSH to suppress their constitutive release under both in vitro and in vivo circumstances. It is the objective of this communication to characterize in greater detail the modulatory effect (s) exerted by FSH on the elaboration of IGFBPs by granulosa cells from immature, estrogen-primed rats. The ability of FSH to regulate the elaboration of granulosa cell-derived IGFBPs proved dose-dependent but biphasic in nature. Specifically, FSH concentrations in the range of 1-3 ng/ml applied for 72 h produced a significant (p < 0.05) increase in polyethylene glycol-precipitable [I-125]IGF-I binding activity corresponding to all IGFBP species detectable by ligand blotting. In contrast, treatment with higher concentrations (greater-than-or-equal-to 10 ng/ml) of FSH resulted in progressive dose-dependent inhibition of the constitutive release of IGF-I binding activity (80% inhibition at the 100 ng/ml dose level) and the virtual elimination of all detectable IGFBP species. Time-course studies disclosed a significant (P < 0.05) initial (apparent at the 24-h time point) stimulatory effect of a high dose of FSH (100 ng/ml) corresponding mostly (but not solely) to the single minor (23K) IGFBP band. In contrast, more prolonged exposure to FSH (greater-than-or-equal-to 48 h) produced progressive time-dependent decrements in IGF-I binding activity, an effect associated with a decrease in the relative representation of the major band doublet (28-29K), the 23K species being all but eliminated under these experimental circumstances. Hypophysectomy produced significant (P < 0.05) inhibition of the subsequent in vitro release of granulosa cell-derived precipitable IGF-I binding activity strongly suggesting that (presumptively stimulatory) pituitary principles other than FSH are likely involved int he regulation of granulosa cell IGFBP release and that the trophic influence of these putative agents appears to outweigh the potential disinhibition of FSH hormonal action. Taken together, these findings indicate that the pituitary regulation of granulosa cell-derived IGFBPs is complex and is not FSH-exclusive. Our findings further indicate that the ability of FSH to regulate granulosa cell-derived IGFBPs is dose- and time-dependent but biphasic in nature, an effect characterized by an early low-dose stimulatory effect and a late high-dose inhibitory effect. Although the physiological significance of the above observations remains uncertain, physiological considerations support the speculation that the biphasic nature or FSH hormonal action may play a role in determining the pace of follicular development and possibly the establishment of follicular dominance.
引用
收藏
页码:754 / 760
页数:7
相关论文
共 33 条
  • [1] FOLLICLE-STIMULATING-HORMONE INHIBITS THE CONSTITUTIVE RELEASE OF INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS BY CULTURED RAT OVARIAN GRANULOSA-CELLS
    ADASHI, EY
    RESNICK, CE
    HERNANDEZ, ER
    HURWITZ, A
    ROSENFELD, RG
    [J]. ENDOCRINOLOGY, 1990, 126 (02) : 1305 - 1307
  • [2] ADASHI EY, IN PRESS MOL CELL EN
  • [3] THE INSULIN-LIKE GROWTH-FACTORS AND THEIR BINDING-PROTEINS
    BAXTER, RC
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1988, 91 (02): : 229 - 235
  • [4] INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN (IGF-BP) INHIBITION OF GRANULOSA-CELL FUNCTION - EFFECT ON CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE, DEOXYRIBONUCLEIC-ACID SYNTHESIS, AND COMPARISON WITH THE EFFECT OF AN IGF-I ANTIBODY
    BICSAK, TA
    SHIMONAKA, M
    MALKOWSKI, M
    LING, N
    [J]. ENDOCRINOLOGY, 1990, 126 (04) : 2184 - 2189
  • [5] SOMATOMEDIN (INSULIN-LIKE GROWTH FACTORS)-BINDING PROTEINS - MOLECULAR-FORMS AND REGULATION
    BINOUX, M
    HOSSENLOPP, P
    HARDOUIN, S
    SEURIN, D
    LASSARRE, C
    GOURMELEN, M
    [J]. HORMONE RESEARCH, 1986, 24 (2-3) : 141 - 151
  • [6] BUSBY WH, 1988, J BIOL CHEM, V263, P14203
  • [7] CHARACTERIZATION OF A PROTEIN IN MID-TERM HUMAN AMNIOTIC-FLUID WHICH REACTS IN SOMATOMEDIN-C RADIORECEPTOR ASSAY
    CHOCHINOV, RH
    MARIZ, IK
    HAJEK, AS
    DAUGHADAY, WH
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1977, 44 (05) : 902 - 908
  • [8] ALTERATIONS IN THE SYNTHESIS OF A FIBROBLAST SURFACE ASSOCIATED 35-K PROTEIN MODULATES THE BINDING OF SOMATOMEDIN-C INSULIN-LIKE GROWTH FACTOR-I
    CLEMMONS, DR
    HAN, VKM
    ELGIN, RG
    DERCOLE, AJ
    [J]. MOLECULAR ENDOCRINOLOGY, 1987, 1 (05) : 339 - 347
  • [9] CULTURED FIBROBLAST MONOLAYERS SECRETE A PROTEIN THAT ALTERS THE CELLULAR-BINDING OF SOMATOMEDIN-C INSULIN-LIKE GROWTH FACTOR-I
    CLEMMONS, DR
    ELGIN, RG
    HAN, VKM
    CASELLA, SJ
    DERCOLE, AJ
    VANWYK, JJ
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1986, 77 (05) : 1548 - 1556
  • [10] GROWTH-HORMONE INCREASES OVARIAN LEVELS OF IMMUNOREACTIVE SOMATOMEDIN-C INSULIN-LIKE GROWTH FACTOR-I INVIVO
    DAVOREN, JB
    HSUEH, AJW
    [J]. ENDOCRINOLOGY, 1986, 118 (02) : 888 - 890