PARTIAL CHARACTERIZATION OF 4 FORMS OF IMMUNOREACTIVE GONADOTROPIN-RELEASING-HORMONE IN THE BRAIN AND TERMINAL NERVE OF THE SPINY DOGFISH (ELASMOBRANCHII, SQUALUS-ACANTHIAS)

Four forms of immunoreactive GnRH have been detected in tissue extracts of both whole brains and terminal nerves from the spiny dogfish (Squalus acanthias). The GnRH forms were characterized using reverse-phase high pressure liquid chromatography (HPLC) and immunological recognition with four different antisera. Three of these forms possess immunological and chromatographic properties consistent with known forms of GnRH: mammalian GnRH, chicken GnRH-II and salmon GnRH. An additional form, with an HPLC elution position intermediate between chicken GnRH-II and salmon GnRH appears to be a new structure of GnRH. The presence of all four GnRH forms in the terminal nerve suggests a lack of regional specificity of the expressed forms of GnRH in the brain.