LOCALIZATION OF ENDOTHELIN RECEPTORS IN THE HUMAN PROSTATE

被引:74
作者
KOBAYASHI, S
TANG, R
WANG, B
OPGENORTH, T
STEIN, E
SHAPIRO, E
LEPOR, H
机构
[1] MED COLL WISCONSIN,DEPT UROL,MILWAUKEE,WI 53226
[2] MED COLL WISCONSIN,DEPT PHARMACOL & TOXICOL,MILWAUKEE,WI 53226
[3] MED COLL WISCONSIN,DEPT PSYCHIAT & MENTAL HLTH,MILWAUKEE,WI 53226
[4] ABBOTT LABS,DIV PHARMACEUT PROD,ABBOTT PK,IL 60064
关键词
RECEPTORS; ENDOTHELIN; MUSCLE; SMOOTH; PROSTATE; PHARMACOLOGY;
D O I
10.1016/S0022-5347(17)35083-8
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
The objective of the present study was to localize endothelin receptors in the human prostate using quantitative autoradiography. Slide-mounted tissue sections 20 mu m. in thickness were obtained from the transition zones of seven patients undergoing radical prostatectomies for low volume prostate cancer. Sarafotoxin (S6C) and BQ123 have been used to distinguish endothelin receptor subtypes (ET(A) and ET(B)). The prostatic tissue sections were incubated in four different stock solutions containing the following: 0.1 nM. I-125-endothelin-1 (I-125-ET-1) (total ET-1 binding); 0.1 nM. I-125-ET-1 and 100 nM. S6C (total ET(A) binding); 0.1 nM. I-125-ET-1 and 1 mu M. BQ123 (total ET(B) binding); and 0.1 nM. I-125-ET-1 and 1 mu M. ET-1 (nonspecific ET-1 binding). Nonspecific binding accounted for only 12 and 15% of total I-125-ET-1 binding in the stroma and glandular epithelium. Autoradiograms were quantitatively analyzed using a computerized image analysis system. Specific radioactive densities (nCi/mg.) were determined for the stromal and glandular epithelial elements of the prostate. The specific radioactive densities of ET(A) and ET(B) binding sites in the stroma were 7.57 +/- 0.65 and 2.98 +/- 0.81. The specific radioactive densities of ET(A) and ET(B) binding sites in the glandular epithelium were 1.59 +/- 0.15 and 7.87 +/- 1.35. The present study demonstrates that the predominant endothelin receptors in the stroma and glandular epithelium are the ET(A) and ET(B) subtypes, respectively.
引用
收藏
页码:763 / 766
页数:4
相关论文
共 26 条
[1]   CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR [J].
ARAI, H ;
HORI, S ;
ARAMORI, I ;
OHKUBO, H ;
NAKANISHI, S .
NATURE, 1990, 348 (6303) :730-732
[2]   TISSUE-SPECIFICITY OF ENDOTHELIN BINDING-SITES [J].
BOLGER, GT ;
LIARD, F ;
KROGSRUD, R ;
THIBEAULT, D ;
JARAMILLO, J .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1990, 16 (03) :367-375
[3]  
Bylund D.B., 1990, METHODS NEUROTRANSMI, P1
[4]   CONTRACTILE EFFECTS OF ENDOTHELIN-1 AND LOCALIZATION OF ENDOTHELIN BINDING-SITES IN RABBIT LOWER URINARY-TRACT SMOOTH-MUSCLE [J].
GARCIAPASCUAL, A ;
LARSSON, B ;
ANDERSSON, KE .
ACTA PHYSIOLOGICA SCANDINAVICA, 1990, 140 (04) :545-555
[5]   BIOLOGICAL PROFILES OF HIGHLY POTENT NOVEL ENDOTHELIN ANTAGONISTS SELECTIVE FOR THE ETA RECEPTOR [J].
IHARA, M ;
NOGUCHI, K ;
SAEKI, T ;
FUKURODA, T ;
TSUCHIDA, S ;
KIMURA, S ;
FUKAMI, T ;
ISHIKAWA, K ;
NISHIKIBE, M ;
YANO, M .
LIFE SCIENCES, 1992, 50 (04) :247-255
[6]   THE HUMAN ENDOTHELIN FAMILY - 3 STRUCTURALLY AND PHARMACOLOGICALLY DISTINCT ISOPEPTIDES PREDICTED BY 3 SEPARATE GENES [J].
INOUE, A ;
YANAGISAWA, M ;
KIMURA, S ;
KASUYA, Y ;
MIYAUCHI, T ;
GOTO, K ;
MASAKI, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2863-2867
[7]   POSITIVE CHRONOTROPIC EFFECTS OF ENDOTHELIN, A NOVEL ENDOTHELIUM-DERIVED VASOCONSTRICTOR PEPTIDE [J].
ISHIKAWA, T ;
YANAGISAWA, M ;
KIMURA, S ;
GOTO, K ;
MASAKI, T .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1988, 413 (01) :108-110
[8]  
KOBAYASHI S, 1993, FASEB J, V7, pA35
[9]  
KUHAR MJ, 1990, METHODS NEUROTRANSMI, P177
[10]  
LANGENSTROER P, 1993, J UROLOGY, V149, P495