IDENTIFICATION OF A MONOCLONAL-ANTIBODY REACTIVE WITH THE BOVINE ORTHOLOGUE OF CD3 (BOCD3)

被引：78

作者：

DAVIS, WC ^{[1
]}

MACHUGH, ND ^{[1
]}

PARK, YH ^{[1
]}

HAMILTON, MJ ^{[1
]}

WYATT, CR ^{[1
]}

机构：

[1] INT LAB RES ANIM DIS,NAIROBI,KENYA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1993年 / 39卷 / 1-3期

关键词：

D O I：

10.1016/0165-2427(93)90167-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A monoclonal antibody (mAb), MM1A, that identifies a molecule expressed on a large percentage of bovine lymphocytes (60-80%) was examined to determine its specificity. Two and three colour immunofluorescence analysis using flow cytometry revealed the molecule is highly expressed on all CD4+ and CD8+ lymphocytes and lymphocytes that express WC1 and the gamma/delta TCR. In contrast to mAbs reactive with BoCD5, MM1A did not react with B lymphocytes. Biochemical analysis revealed that the mAb immunoprecipitates a molecular complex comprising a set of peptides with M(r) approximately 12, 16, 22, 32, 36, and 44 kDa under reducing conditio ns and an additional 96 kDa peptide complex under non-reducing conditions. The data indicate that MM1A recognizes the bovine orthologue of CD3 (BoCD3).

引用

页码：85 / 91

页数：7

共 15 条

[1] GENETIC AND MUTATIONAL ANALYSIS OF THE T-CELL ANTIGEN RECEPTOR [J].