POTENTIOMETRIC DETERMINATION OF CARBONIC-ANHYDRASE ACTIVITY IN RABBIT CAROTID-BODIES - COMPARISON AMONG NORMOXIC, HYPEROXIC AND HYPOXIC ANIMALS

被引：7

作者：

BOTRE, F

BOTRE, C

GRECO, A

DATA, PG

DIGIULIO, C

MORELLI, L

机构：

[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO STUDI CHIM & TECNOL SOSTANCE BIOL AT,I-00185 ROME,ITALY

[2] UNIV G DANNUNZIO,IST FISIOL,I-66100 CHIETI,ITALY

来源：

NEUROSCIENCE LETTERS | 1994年 / 166卷 / 02期

关键词：

CARBONIC ANHYDRASE; CAROTID BODY; HYPEROXIA; HYPOXIA; POTENTIOMETRIC DETERMINATION; CO2; SENSOR;

D O I：

10.1016/0304-3940(94)90467-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The catalytic activity of carbonic anhydrase (CA) contained in the glomus cells of mammalian carotid bodies has been determined in vitro by a potentiometric method. Experiments performed on whole rabbit carotid bodies have shown a very low variability, in terms of the overall CA activity, among organs belonging to different animals maintained in normoxic conditions. Repeated assays performed on each carotid body have shown a marked decrease of the overall CA activity after the first assay, thus suggesting the presence of at]east two different forms of enzyme. Experiments performed on carotid bodies belonging to rabbits maintained in normal, hyperoxic and hypoxic conditions have shown that the overall CA activity follows the sequence: hypoxic > normoxic > hyperoxic, matching with the corresponding physiological activity of the carotid body.

引用

页码：126 / 130

页数：5

共 17 条

[1]

Balentine J.D., 1982, PATHOLOGY OXYGEN TOX

[2] RESPONSES OF CAROTID BODY CHEMORECEPTORS IN CAT TO SUDDEN CHANGES OF HYPERCAPNIC AND HYPOXIC STIMULI [J].