DETERMINATION OF NONPROTEIN AMINO-ACIDS AND TOXINS IN LATHYRUS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PRECOLUMN PHENYL ISOTHIOCYANATE DERIVATIZATION

被引：50

作者：

KHAN, JK ^{[1
]}

KUO, YH ^{[1
]}

KEBEDE, N ^{[1
]}

LAMBEIN, F ^{[1
]}

机构：

[1] STATE UNIV GHENT,FAC MED,PHYSIOL CHEM LAB,B-9000 GHENT,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 687卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)00777-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A simple procedure for the precolumn derivatization of toxic and non-toxic non-protein amino acids occurring in the legume crop Lathyrus sativus and other Lathyrus species with phenyl isothiocyanate and an HPLC method for the separation of the derivatives in the nano- and picomole range are reported. The results are compared with those of conventional ion-exchange chromatography for the separation of non-protein amino acids with postcolumn ninhydrin reaction. The relative standard deviations for the two methods are compared.

引用

页码：113 / 119

页数：7

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