DNA-BASED TYPING OF HUMAN PLATELET ANTIGEN SYSTEMS BY POLYMERASE CHAIN REACTION-SINGLE-STRAND CONFORMATION POLYMORPHISM METHOD

被引：15

作者：

FUJIWARA, K ^{[1
]}

TOKUNAGA, K ^{[1
]}

ISA, K ^{[1
]}

MIYAMOTO, M ^{[1
]}

WANG, L ^{[1
]}

AKAZA, T ^{[1
]}

TADOKORO, K ^{[1
]}

SHIBATA, Y ^{[1
]}

JUJI, T ^{[1
]}

机构：

[1] UNIV TOKYO, FAC MED, DEPT TRANSFUS MED & IMMUNOHEMATOL, TOKYO, JAPAN

来源：

VOX SANGUINIS | 1995年 / 69卷 / 04期

关键词：

D O I：

10.1111/j.1423-0410.1995.tb00371.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) method has been established to discriminate genotypes for the human platelet antigen (HPA) systems HPA-1, HPA-2, HPA-3, HPA-4, and HPA-5. Gene fragments which contain polymorphic sequences corresponding to the HPA-1, HPA-2 HPA-3, HPA-4, and HPA-5 systems were PCR-amplified with specific primers. The amplified DNA was denatured and subjected to non-denaturing polyacrylamide gel electrophoresis followed by silver staining. The results obtained by the PCR-SSCP method were in good agreement with those of the allotypes determined by serological typing. Furthermore, the results agreed with those obtained by other DNA-based typing methods such as PCR-allele-specific restriction enzyme analysis and PCR-sequence-specific primer. These results indicate that PCR-SSCP is a simple and sensitive method for determining HPA genotypes and identifying unknown polymorphisms.

引用

页码：347 / 351

页数：5

共 26 条

[1]

von dem Borne, van Leeuwen EF, von Riesz LE, van Boxtel CJ, Engelfriet CP, Neonatal alloimmune thrombocytopenia: Detection and characterization of the responsible antibodies by the platelet immunofluorescence test, Blood, 57, pp. 649-656, (1981)

[2]

Lau P., Sholtis CM, Aster RH, Post‐transfusion purpura: An enigma of alloimmunization, Am J Hematol, 9, pp. 331-336, (1980)

[3]

Shibata Y., Miyaji T., Ichikawa Y., Matsuda I., A new platelet antigen system, Yuka/Yukb, Vox Sang, 51, pp. 334-336, (1986)

[4]

Saji H., Maruya E., Fujii H., Maekawa T., Akiyama Y., Matsuura T., Hosoi T., New platelet antigen, Siba, involved in platelet transfusion refractoriness in Japanese man, Vox Sang, 56, pp. 283-287, (1989)

[5]

Kunicki TJ, Newman PJ, The molecular immunology of platelet proteins, Blood, 80, pp. 1386-1404, (1992)

[6]

Newman PJ, Derbes RS, Aster RH, The human platelet alloantigens, P1A1 and PlA2, are associated with a leucine33/proline33 amino acid polymorphism in membrane glycoprotein IIIa, and are distinguishable by DNA typing, J Clin Invest, 83, pp. 1778-1781, (1989)

[7]

Kuijpers RWAM, Faber NM, Cuypers HTM, Ouwehand WH, von dem Borne, NH2‐terminal globular domain of human platelet glycoprotein lbα(has a methionine 145/threonine145 amino acid polymorphism, which is associated with the HPA‐2 (Ko) alloantigens, J Clin Invest, 8, pp. 381-384, (1992)

[8]

Lyman S., Aster RH, Visentin GP, Newman PJ, Polymorphism of human platelet membrane glycoprotein IIb associated with the Baka/Bakb alloantigen system, Blood, 75, pp. 2343-2348, (1990)

[9]

Wang L., Juji T., Shibata Y., Kuwata S., Tokunaga K., Sequence variation of human platelet membrane glycoprotein IIIa associated with the Yuka/Yukb alloantigen system, Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences, 67, pp. 102-106, (1991)

[10]

Santoso S., Kalb R., Walka M., Kiefel V., Mueller-Eckhardt C., Newman PJ, The human platelet alloantigens Bra and Brb are associated with a single aminoacid polymorphism on glycoprotein Ia (integrin subunit α2), J Clin Invest, 92, pp. 2427-2432, (1993)

← 1 2 3 →