3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE FROM THE LATEX OF HEVEA-BRASILIENSIS

被引：14

作者：

WITITSUWANNAKUL, R ^{[1
]}

WITITSUWANNAKUL, D ^{[1
]}

SUWANMANEE, P ^{[1
]}

机构：

[1] MAHIDOL UNIV, FAC SCI, DEPT BIOCHEM, BANGKOK 10400, THAILAND

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 05期

关键词：

Euphorbiaceae; Hevea brasiliensis; HMG-CoA reductase; properties; purification; rubber latex;

D O I：

10.1016/0031-9422(90)80089-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3-Hydroxy-3-methylglutaryl coenzyme A reductase (mevalonate: NADP+ oxidoreductase acylating CoA; EC 1.1.1.34) was purified from fresh Hevea latex of clone RRIM 600. The latex was centrifuged and the sediment used for enzyme purification. The enzyme was solubilized by freeze-thawing in a buffer containing 1 % Brij W-1 and 20% glycerol. Affinity chromatography (HMG-CoA-Hexyl-Agarose) was used in the final purification step. The Mr determined by SDS-PAGE was 44000 and that estimated from non-denaturing gel electrophoresis, 176000. The optimal pH was ca 7, with an apparent Km of 13 μM for (S)-HMG-CoA. A low concentration of dithiothreitol was required for maximal activity of the purified enzyme. © 1990.

引用

页码：1401 / 1403

页数：3

共 25 条

[1]

BACH TJ, 1987, PLANT PHYSIOL BIOCH, V25, P163

[2] DETERGENT-SOLUBILIZATION, PURIFICATION, AND CHARACTERIZATION OF MEMBRANE-BOUND 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE FROM RADISH SEEDLINGS [J].

BACH, TJ ;

ROGERS, DH ;

RUDNEY, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 154 (01) :103-111

[3]

BENEDICT CR, 1982, BIOSYNTHESIS ISOPREN, V2, P356

[4] REGULATION OF MICROSOMAL 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE FROM PEA-SEEDLINGS - RAPID POSTTRANSLATIONAL PHYTOCHROME-MEDIATED DECREASE IN ACTIVITY AND INVIVO REGULATION BY ISOPRENOID PRODUCTS [J].

BROOKER, JD ;

RUSSELL, DW .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1979, 198 (01) :323-334

[5]

BROWN WE, 1980, DEHYDROGENASES REQUI, P232

[6] NUCLEOTIDE-SEQUENCE OF 3-HYDROXY-3-METHYL-GLUTARYL COENZYME-A REDUCTASE, A GLYCOPROTEIN OF ENDOPLASMIC-RETICULUM [J].

CHIN, DJ ;

GIL, G ;

RUSSELL, DW ;

LISCUM, L ;

LUSKEY, KL ;

BASU, SK ;

OKAYAMA, H ;

BERG, P ;

GOLDSTEIN, JL ;

BROWN, MS .

NATURE, 1984, 308 (5960) :613-617

[7] AMO 1618 EFFECTS ON INCORPORATION OF MVA-C-14 AND ACETATE-C-14 INTO STEROLS IN NICOTIANA AND DIGITALIS SEEDLINGS AND CELL-FREE PREPARATIONS FROM NICOTIANA [J].

DOUGLAS, TJ ;

PALEG, LG .

PHYTOCHEMISTRY, 1978, 17 (04) :713-718

[8]

Gark VK, 1983, 3 HYDROXY 3 METHYLGL, P29

[9] METHOD FOR DETERMINING REDUCTASE-ACTIVITY OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A IN RAT LIVERS [J].

HAMPRECHT, B ;

LYNEN, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 14 (02) :323-+

[10] BIOSYNTHESIS OF RUBBER FROM BETA-HYDROXY-BETA-METHYLGLUTARYL-COENZYME A IN HEVEA BRASILIENSIS LATEX [J].

HEPPER, CM ;

AUDLEY, BG .

BIOCHEMICAL JOURNAL, 1969, 114 (02) :379-&

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