DNA WITHOUT SUPERTWISTS CAN BE AN INVITRO SUBSTRATE FOR SITE-SPECIFIC RECOMBINATION OF BACTERIOPHAGE-LAMBDA

被引：33

作者：

POLLOCK, TJ ^{[1
]}

ABREMSKI, K ^{[1
]}

机构：

[1] NCI, MOLEC BIOL LAB, BETHESDA, MD 20205 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1979年 / 131卷 / 03期

关键词：

D O I：

10.1016/0022-2836(79)90013-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A negatively supertwisted substrate is required for site-specific recombination of baeteriophage λ in vitro under conditions of high ionic strength. This requirement is eliminated under conditions of low ionic strength. Both integrative (attB × attP) and excisive (attR × attL) recombinations display this property. Either nicked or hydrogen-bonded circular forms of DNA recombine in low ionic strength environments in the absence of DNA gyrase activity. © 1979.

引用

页码：651 / 654

页数：4

共 14 条

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