RAT-KIDNEY 25-HYDROXYVITAMIN D(3) 1-ALPHA-HYDROXYLASES AND 24-HYDROXYLASES - EVIDENCE FOR 2 DISTINCT GENE-PRODUCTS

被引：4

作者：

ARABIAN, A

GROVER, J

BARRE, MG

DELVIN, EE

机构：

[1] SHRINERS HOSP CRIPPLED CHILDREN, GENET UNIT, 1529 CEDAR AVE, MONTREAL H3G 1A6, PQ, CANADA

[2] HOP ST LUC, CTR RECH CLIN ANDRE VIALLET, MONTREAL H2X 3J4, QUEBEC, CANADA

[3] MCGILL UNIV, DEPT PHYSIOL, MONTREAL H3A 2T5, QUEBEC, CANADA

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1993年 / 45卷 / 06期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0960-0760(93)90167-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Total RNA was isolated from kidneys of Sprague-Dawley rats. Oligo (dT)-primed single-stranded cDNA was obtained by the reverse transcriptase reaction from which a 285 bp cDNA probe coding for 25-hydroxyvitamin D3-24-hydroxylase [25(OH)D3-24-OHase] was generated by the polymerase chain reaction. Northern blotting performed with kidney poly (A)+ RNA isolated from rats (1) fed a standard diet, (2) depleted in D3 and hypocalcemic, and (3) fed a standard diet and injected intraperitoneally with 50,000 IU of vitamin D3 for 5 days showed that the transcript for 24-OHase was weakly expressed in control, and highly induced in vitamin D3-treated animals. No transcript could be elicited in vitamin D-depleted hypocalcemic animals in which 25(OH)D3-1alpha-OHase was maximally induced. The data show that 24-OHase is independently regulated of 1alpha-OHase, strongly suggestive of the enzymes being encoded by two distinct genes.

引用

页码：513 / 516

页数：4

共 21 条

[1]

ANDERSON MLM, 1985, NUCLEIC ACID HYBRIDI, P73

[2] CHARACTERIZATION OF MONOCLONAL-ANTIBODIES SPECIFIC TO BOVINE RENAL VITAMIN-D HYDROXYLASES [J].