MOLECULAR ANALYSIS OF A LEU2(-) MUTANT OF CANDIDA-MALTOSA DEMONSTRATES THE PRESENCE OF MULTIPLE ALLELES

被引：9

作者：

BECHER, D ^{[1
]}

SCHULZE, S ^{[1
]}

KASUSKE, A ^{[1
]}

SCHULZE, H ^{[1
]}

SAMSONOVA, IA ^{[1
]}

OLIVER, SG ^{[1
]}

机构：

[1] UNIV MANCHESTER,INST SCI & TECHNOL,DEPT BIOCHEM & APPL MOLEC BIOL,MANCHESTER M60 1QD,LANCS,ENGLAND

来源：

CURRENT GENETICS | 1994年 / 26卷 / 03期

关键词：

BETA-ISOPROPYLMALATE DEHYDROGENASE; ALLELIC VARIATION; GENOME ORGANIZATION; CANDIDA MALTOSA; SILENT GENES;

D O I：

10.1007/BF00309549

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Three different alleles of the beta-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy - prototrophy - auxotrophy sequence of mutations in C. maltosa is suggested.

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页码：208 / 216

页数：9

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