MOLECULAR ANALYSIS OF A LEU2(-) MUTANT OF CANDIDA-MALTOSA DEMONSTRATES THE PRESENCE OF MULTIPLE ALLELES

被引:9
作者
BECHER, D [1 ]
SCHULZE, S [1 ]
KASUSKE, A [1 ]
SCHULZE, H [1 ]
SAMSONOVA, IA [1 ]
OLIVER, SG [1 ]
机构
[1] UNIV MANCHESTER,INST SCI & TECHNOL,DEPT BIOCHEM & APPL MOLEC BIOL,MANCHESTER M60 1QD,LANCS,ENGLAND
关键词
BETA-ISOPROPYLMALATE DEHYDROGENASE; ALLELIC VARIATION; GENOME ORGANIZATION; CANDIDA MALTOSA; SILENT GENES;
D O I
10.1007/BF00309549
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Three different alleles of the beta-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy - prototrophy - auxotrophy sequence of mutations in C. maltosa is suggested.
引用
收藏
页码:208 / 216
页数:9
相关论文
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[21]  
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