NEW CCDB POSITIVE-SELECTION CLONING VECTORS WITH KANAMYCIN OR CHLORAMPHENICOL SELECTABLE MARKERS

被引：40

作者：

BERNARD, P ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,DEPT BIOL MOLEC,GENET LAB,B-1640 RHODE ST GENESE,BELGIUM

来源：

GENE | 1995年 / 162卷 / 01期

关键词：

HIGH-COPY-NUMBER PUC18/19; PLASMIDS; MULTIPLE CLONING SITE; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(95)00314-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Recently, we described pKIL18/19 positive-selection cloning vectors which rely on the inactivation of the cytotoxic ccdB gene [Bernard et al., Gene 148 (1994) 71-74]. They are new tools for simplifying gene cloning/sequencing procedures, as only recombinant DNA allow the formation of viable colonies. To enhance positive-selection capabilities, putative translational reinitiation codons located within the pKIL18/19 ccdB were modified by site-directed mutagenesis. New pKIL-derived vectors with kanamycin (Km(R)) or chloramphenicol (Cm-R) resistance-encoding genes were also constructed. In addition, a new host derived from the DH2 strain was developed.

引用

页码：159 / 160

页数：2

共 6 条

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