SEPARATION OF BASAL AND DIFFERENTIATING CELLS FROM HUMAN-EPIDERMIS FOR DNA CYTOFLUOROMETRY

被引：12

作者：

SASAI, Y

KAWAMURA, K

NAMBA, K

机构：

[1] Department of Dermatology, Kurume University School of Medicine, Kurume, 830

来源：

HISTOCHEMISTRY | 1979年 / 63卷 / 03期

关键词：

D O I：

10.1007/BF00490055

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A method for the selective separation of human epidermal cells, in which the Feulgen-stainable material suffers minimal damage, was investigated. The principal stage involves α-chymotrypsin treatment of skin specimens as well as shaking in an isotonic solution. With respect to DNA distribution pattern, there was good agreement between that of epidermal cells separated with the microdissection-ultrasonic irradiation method, reported previously by us, and those separated by the present method. © 1979 Springer-Verlag.

引用

页码：265 / 272

页数：8

共 15 条

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