PARTIAL-PURIFICATION OF A PEA SEED DNA-BINDING PROTEIN THAT SPECIFICALLY RECOGNIZES 5-METHYLCYTOSINE

被引：6

作者：

EHRLICH, KC

机构：

[1] Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, LA

来源：

PREPARATIVE BIOCHEMISTRY | 1993年 / 23卷 / 04期

关键词：

D O I：

10.1080/10826069308544567

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, a DNA-binding protein (DBPm) was identified in plant nuclei that may mediate the effects of DNA methylation on chromatin structure and transcription. In the present report, DBPm was partially purified from germinated pea (Pisum sativum) seed nuclear extracts by DEAE-cellulose, phenylsepharose, heparin-sepharose chromatography, and preparative mobility shift on polyacrylamide gels. The purified activity showed a band at approximately 50 kD by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as well as by Sephadex 0100 chromatography, suggesting that DBPm is present as a monomer.

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页码：423 / 438

页数：16

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