COORDINATED EXPRESSION AND FUNCTION OF NEUROTROPHINS AND THEIR RECEPTORS IN THE RAT INNER-EAR DURING TARGET INNERVATION

被引:181
作者
PIRVOLA, U
ARUMAE, U
MOSHNYAKOV, M
PALGI, J
SAARMA, M
YLIKOSKI, J
机构
[1] UNIV HELSINKI,INST BIOTECHNOL,HELSINKI,FINLAND
[2] ESTONIAN ACAD SCI,INST CHEM PHYS & BIOPHYS,TALLINN,ESTONIA
[3] UNIV KUOPIO,DEPT OTOLARYNGOL,SF-70211 KUOPIO,FINLAND
关键词
NEUROTROPHIC SYSTEM; NEURITOGENESIS; NEURONAL SURVIVAL; COCHLEOVESTIBULAR GANGLION; OTIC VESICLE;
D O I
10.1016/0378-5955(94)90064-7
中图分类号
R36 [病理学]; R76 [耳鼻咽喉科学];
学科分类号
100104 ; 100213 ;
摘要
We show that trkB and trkC mRNAS, encoding the high-affinity receptor tyrosine kinases for brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), respectively, as well as low-affinity nerve growth factor receptor (p75(LNGFR)) mRNA are expressed in the cochleovestibular ganglion (CVG) before and during innervation of the target fields. Correspondingly, from preinnervation stages onward, BDNF and NT-3, but neither nerve growth factor (NGF)nor neurotrophin-4(NT-4) mRNAs are expressed in the sensory epithelium of the otic vesicle, the peripheral target field of CVG neurons. No neurotrophin transcripts were detected by in situ hybridization in the medullary central targets. In explant cultures, neuritogenesis from both the cochlear and vestibular part of the CVG was promoted by BDNF, while NT-3 evoked neurites mainly from the cochlear neurons. Also NT-4 stimulated neurite outgrowth from the CVG in vitro. In dissociated neuron-enriched cultures, NT-3 and BDNF promoted survival of overlapping subsets of CVG neurons and, correspondingly, results from in situ hybridization showed that both trkC and trkB mrnas were expressed in most neurons of this ganglion. The negligible effect of NGF seen in the bioassays agrees well with the expression of only a few trkA transcripts, encoding the high-affinity receptor for NGF, in the CVG. Based on the spatiotemporal expression patterns and biological effects in vitro, peripherally-synthesized BDNF and NT-3 regulate the survival of CVG neurons as well as the establishment of neuron-target cell contacts in the early-developing inner ear. In addition, the expression of trkB mRNA, more specifically its truncated form, and trkC as well as p75(LNGFR) mRNAS in distinct, non-neuronal structures indicates novel roles for these molecules during development.
引用
收藏
页码:131 / 144
页数:14
相关论文
共 56 条
[1]  
ALTMAN J, 1982, ADV ANAT EMBRYOL CEL, V74, P1
[2]   TROPHIC INTERACTIONS BETWEEN THE COCHLEO-VESTIBULAR GANGLION OF THE CHICK-EMBRYO AND ITS SYNAPTIC TARGETS IN CULTURE [J].
ARD, MD ;
MOREST, DK ;
HAUGER, SH .
NEUROSCIENCE, 1985, 16 (01) :151-170
[3]   NEUROTROPHINS AND THEIR RECEPTORS IN RAT PERIPHERAL TRIGEMINAL SYSTEM DURING MAXILLARY NERVE GROWTH [J].
ARUMAE, U ;
PIRVOLA, U ;
PALGI, J ;
KIEMA, TR ;
PALM, K ;
MOSHNYAKOV, M ;
YLIKOSKI, J ;
SAARMA, M .
JOURNAL OF CELL BIOLOGY, 1993, 122 (05) :1053-1065
[4]   TROPHIC FACTORS AND NEURONAL SURVIVAL [J].
BARDE, YA .
NEURON, 1989, 2 (06) :1525-1534
[5]   PURIFICATION OF A NEW NEUROTROPHIC FACTOR FROM MAMMALIAN BRAIN [J].
BARDE, YA ;
EDGAR, D ;
THOENEN, H .
EMBO JOURNAL, 1982, 1 (05) :549-553
[6]   NEUROTROPHIN-5 - A NOVEL NEUROTROPHIC FACTOR THAT ACTIVATES TRK AND TRKB [J].
BERKEMEIER, LR ;
WINSLOW, JW ;
KAPLAN, DR ;
NIKOLICS, K ;
GOEDDEL, DV ;
ROSENTHAL, A .
NEURON, 1991, 7 (05) :857-866
[7]   CHARACTERIZATION AND LOCALIZATION OF NERVE GROWTH-FACTOR RECEPTORS IN THE EMBRYONIC OTIC VESICLE AND COCHLEO-VESTIBULAR GANGLION [J].
BERND, P ;
REPRESA, J .
DEVELOPMENTAL BIOLOGY, 1989, 134 (01) :11-20
[8]  
BUCHMAN VL, 1993, DEVELOPMENT, V118, P989
[9]   TEMPORAL PATTERNS OF NEUROGENESIS IN AVIAN CRANIAL SENSORY AND AUTONOMIC GANGLIA [J].
DAMICOMARTEL, A .
AMERICAN JOURNAL OF ANATOMY, 1982, 163 (04) :351-372
[10]  
DAVIES AM, 1986, J NEUROSCI, V6, P1897