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PRINS AS A METHOD FOR RAPID CHROMOSOMAL LABELING ON HUMAN SPERMATOZOA

被引：22
作者：
PELLESTOR, F
GIRARDET, A
LEFORT, G
ANDREO, B
CHARLIEU, JP
机构：
[1] ST CHARLES HOSP,MONTPELLIER,FRANCE
[2] WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
来源：
MOLECULAR REPRODUCTION AND DEVELOPMENT | 1995年 / 40卷 / 03期
关键词：
HUMAN SPERM; PRINS; ANEUPLOIDY; CHROMOSOMES; 13; 16; AND; 21;
D O I：
10.1002/mrd.1080400309
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Direct in situ labeling of human spermatozoa was performed using the PRINS method. This technique is based on annealing of specific oligonucleotide primers, and subsequent primer extension by a Taq DNA polymerase. The reaction was carried out on a programmable temperature cycler, and labeling was obtained in a 1-hr reaction. The method was successfully tested with specific primers for chromosomes 13, 16, and 21. This suggests that PRINS may be a fast and reliable technique for detecting aneuploidies. (C) 1995 Wiley-Liss, Inc.
引用
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页码：333 / 337
页数：5
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