STRUCTURE OF BOTH THE LIGAND AND LIPID-DEPENDENT CHANNEL-INACTIVE STATES OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR PROBED BY FTIR SPECTROSCOPY AND HYDROGEN EXCHANGES

被引:46
作者
METHOT, N [1 ]
DEMERS, CN [1 ]
BAENZIGER, JE [1 ]
机构
[1] UNIV OTTAWA,DEPT BIOCHEM,OTTAWA,ON K1H 8M5,CANADA
关键词
D O I
10.1021/bi00046a021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
FTIR spectra have been recorded both as a function of time and after prolonged exposure to (H2O)-H-2 buffer in order to study the structural changes that lead to both the ligand- and lipid-dependent channel-inactive states of the nicotinic acetylcholine receptor (nAChR). The hydrogen/deuterium exchange spectra provide insight into both the overall rates and extent of peptide H-1/H-2 exchange and the individual rates and extent to which peptide hydrogens in alpha-helix and beta-sheet conformations exchange for deuterium. The spectra are also sensitive to the conformation of the polypeptide backbone and thus the secondary structure of the nAChR. The various spectral features monitored in the presence and absence of carbamylcholine and tetracaine are essentially identical, indicating that there are no large net changes in secondary structure in the channel-inactive desensitized state. The various spectral features monitored for the nAChR reconstituted into lipid membranes either with or without cholesterol are very similar, indicating that cholesterol is not a major structural regulator of the nAChR. However, in the absence of both cholesterol and anionic lipids, there is a slightly enhanced rate of exchange of alpha-helical peptide hydrogens for deuterium that occurs as a result of either an increase in nAChR dynamics or an increase in the accessibility of transmembrane peptide hydrogens (H2O)-H-2. The latter may simply be due to an increase in the ''fluidity'' and thus permeability of the lipid bilayers to aqueous solvent. The results indicate that channel inactivation is due to a very subtle change in structure of the nAChR, regardless of whether the inactive state is stabilized by either prolonged exposure to carbamylcholine or reconstitution into lipid membranes lacking cholesterol and anionic lipids. The data also illustrate the sensitivity of the amide I band shape to peptide H-1/H-2 exchange. Sample variations in the extent of peptide H-1/H-2 exchange can lead to changes in the amide I band that are easily misinterpreted in terms of a change in protein secondary structure.
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页码:15142 / 15149
页数:8
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