ARACHIDONIC-ACID EPOXYGENASE - STRUCTURAL CHARACTERIZATION AND QUANTIFICATION OF EPOXYEICOSATRIENOATES IN PLASMA

被引：102

作者：

KARARA, A

WEI, S

SPADY, D

SWIFT, L

CAPDEVILA, JH

FALCK, JR

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT MED NEPHROL,NASHVILLE,TN 37232

[2] VANDERBILT UNIV,MED CTR,SCH MED,DEPT PATHOL,NASHVILLE,TN 37232

[3] SW MED CTR,DEPT MOLEC GENET,DALLAS,TX 75235

[4] SW MED CTR,DEPT INTERNAL MED,DALLAS,TX 75235

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91877-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gas chromatographic/mass spectroscopic and chiral analysis showed the presence of enzymatically derived 8,9-, 11,12- and 14,15-EET in rat plasma (2.8:1:3.4 molar ratio, respectively; 10.2 ± 0.4 ng total EET/ml plasma). Greater than 90% of the plasma EETs was esterified to the phospholipids of circulating lipoproteins. The lipoprotein fraction with the highest EET concentration was LDL (8.1 ± 0.9 ng/mg of protein) followed by HDL and VLDL (3.5 ± 0.1 and 1.9 ± 0.3 ng/mg of protein, respectively). In light of the biological activities of the EETs, these results suggest a potential systemic function for the cytochrome P-450 epoxygenase. © 1992.

引用

收藏

页码：1320 / 1325

页数：6

相关论文

共 20 条

[1] VASOACTIVITY OF ARACHIDONIC-ACID EPOXIDES [J].

CARROLL, MA ;

SCHWARTZMAN, M ;

CAPDEVILA, J ;

FALCK, JR ;

MCGIFF, JC .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1987, 138 (02) :281-283

[2] ENDOGENOUS BIOSYNTHESIS OF ARACHIDONIC-ACID EPOXIDES IN HUMANS - INCREASED FORMATION IN PREGNANCY-INDUCED HYPERTENSION [J].

CATELLA, F ;

LAWSON, JA ;

FITZGERALD, DJ ;

FITZGERALD, GA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5893-5897

[3] OXIDIZED LOW-DENSITY LIPOPROTEIN INDUCES DIFFERENTIATION AND ADHESION OF HUMAN MONOCYTES AND THE MONOCYTIC CELL-LINE U937 [J].

FROSTEGARD, J ;

NILSSON, J ;

HAEGERSTRAND, A ;

HAMSTEN, A ;

WIGZELL, H ;

GIDLUND, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :904-908

[4] OXIDIZED LOW-DENSITY LIPOPROTEINS POTENTIATE VASOCONSTRICTIONS TO VARIOUS AGONISTS BY DIRECT INTERACTION WITH VASCULAR SMOOTH-MUSCLE [J].

GALLE, J ;

BASSENGE, E ;

BUSSE, R .

CIRCULATION RESEARCH, 1990, 66 (05) :1287-1293

[5] MALONDIALDEHYDE-ALTERED PROTEIN OCCURS IN ATHEROMA OF WATANABE HERITABLE HYPERLIPIDEMIC RABBITS [J].

HABERLAND, ME ;

FONG, D ;

CHENG, L .

SCIENCE, 1988, 241 (4862) :215-218

[6] DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM [J].

HAVEL, RJ ;

EDER, HA ;

BRAGDON, JH .

JOURNAL OF CLINICAL INVESTIGATION, 1955, 34 (09) :1345-1353

[7] OXIDATION OF BETA-VERY LOW-DENSITY-LIPOPROTEIN BY ENDOTHELIAL-CELLS ENHANCES ITS METABOLISM BY SMOOTH-MUSCLE CELLS IN CULTURE [J].

HORRIGAN, S ;

CAMPBELL, JH ;

CAMPBELL, GR .

ARTERIOSCLEROSIS AND THROMBOSIS, 1991, 11 (02) :279-289

[8]

KARARA A, 1989, J BIOL CHEM, V264, P19822

[9]

KARARA A, 1991, J BIOL CHEM, V266, P7561

[10] GLOMERULAR STEREOSPECIFIC SYNTHESIS AND HEMODYNAMIC ACTIONS OF 8,9-EPOXYEICOSATRIENOIC ACID IN RAT-KIDNEY [J].

KATOH, T ;

TAKAHASHI, K ;

CAPDEVILA, J ;

KARARA, A ;

FALCK, JR ;

JACOBSON, HR ;

BADR, KF .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (04) :F578-F586