PHOTOPRODUCTION OF HYDROGEN FROM THE WASTE-WATER OF A DISTILLERY BY RHODOBACTER-SPHAEROIDES OU 001

被引:41
作者
SASIKALA, K
RAMANA, CV
RAO, PR
机构
[1] Microbial Biotechnology Laboratory, Department of Botany, Osmania University, Hyderabad
关键词
D O I
10.1016/0360-3199(92)90217-K
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Waste water from a distillery was used as the electron donor for photoproduction of hydrogen by Rhodobacter sphaeroides O.U. 001. Hydrogen production by free cells was observed only at 5 and 10% waste water (diluted with tap water) yielding a maximum of 500-mu-l H-2 (in 144 h) at 10% concentration. Calcium alginate immobilization of cells enhanced hydrogen photoproduction two-three fold and hydrogen evolution was observed after varying lag periods at all the concentrations of waste water tested. Semicontinuous culturing of R. sphaeroides was demonstrated in a 3.61 photobioreactor harvesting 11 of culture every day amounting to 2 g dry wt. Hydrogen production was demonstrated for 12 days using immobilized cells with 10% waste water in a 3.61 photobioreactor under light/dark cycles.
引用
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页码:23 / 27
页数:5
相关论文
共 30 条
[1]  
BOLLIGER R, 1985, APPL MICROBIOL BIOT, V23, P147, DOI 10.1007/BF00938968
[2]  
BURANAKARL L, 1976, AGR BIOL CHEM TOKYO, V49, P3339
[3]   ANAEROBIC AND AEROBIC HYDROGEN GAS FORMATION BY BLUE-GREEN-ALGA ANABAENA-CYLINDRICA [J].
DADAY, A ;
PLATZ, RA ;
SMITH, GD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 34 (05) :478-483
[4]  
FUJII T, 1983, AGR BIOL CHEM TOKYO, V47, P2747
[5]  
GADRE R V, 1986, Indian Journal of Environmental Health, V28, P54
[6]  
GREENBAUM E, 1988, ALGAE HUMAN AFFAIRS, P1
[7]  
KARUBE I, 1988, METHOD ENZYMOL, V137, P668
[8]  
KESSLER E, 1974, ALGAL PHYSL BIOCH, P456
[9]  
KIM JS, 1981, J FERMENT TECHNOL, V59, P185
[10]   HYDROGEN FORMATION IN NEARLY STOICHIOMETRIC AMOUNTS FROM GLUCOSE BY A RHODOPSEUDOMONAS-SPHAEROIDES MUTANT [J].
MACLER, BA ;
PELROY, RA ;
BASSHAM, JA .
JOURNAL OF BACTERIOLOGY, 1979, 138 (02) :446-452