TEMPERATURE-SENSITIVE INTERMEDIATE FILAMENT ASSEMBLY ALTERNATIVE STRUCTURES OF XENOPUS-LAEVIS VIMENTIN IN-VITRO AND IN-VIVO

被引:60
作者
HERRMANN, H
ECKELT, A
BRETTEL, M
GRUND, C
FRANKE, WW
机构
[1] Division of Cell Biology, German Cancer Research Center, D-69120 Heidelberg
关键词
INTERMEDIATE FILAMENTS; VIMENTIN; FILAMENT ASSEMBLY; NUCLEAR TRANSPORT;
D O I
10.1006/jmbi.1993.1566
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In assembly assays of intermediate filaments (IFs) from vimentin of the amphibian species Xenopus laevis we have observed the formation of so far unknown structures at temperatures above 28°C. Upon assembly in vitro at temperatures above 34°C massive aggregates, partly with a protofilamentous substructure, were found and their formation correlated with drastically reduced end-viscosity. Large spheroidal, dense aggregates with a complex suborganization were also seen to form at 37°C in the cytoplasm of living mammalian cells devoid of endogenous vimentin upon transfection with cDNA encoding the amphibian vimentin, and this was also true for vimentin forced to accumulate in the nucleoplasm by the introduction of a “nuclear localization signal”. Upon shift from the non-permissive (37°C) to the permissive (28°C) temperature, such aggregates of non-IF vimentin structures gradually disappeared and a normal-looking IF meshwork formed. The results, which are discussed in relation to other structures assembled by IF proteins, indicate a marked thermosensitivity in the amino acid sequence of the vimentin which seems to have been reduced during evolution of warm-blooded animals. They further show that members of the multigene gene family of IF proteins can occur in structures totally different from IFs. © 1993 Academic Press Limited.
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页码:99 / 113
页数:15
相关论文
共 67 条
[1]   THE FIBRILLAR SUBSTRUCTURE OF KERATIN FILAMENTS UNRAVELED [J].
AEBI, U ;
FOWLER, WE ;
REW, P ;
SUN, TT .
JOURNAL OF CELL BIOLOGY, 1983, 97 (04) :1131-1143
[2]   UNIFYING PRINCIPLES IN INTERMEDIATE FILAMENT (IF) STRUCTURE AND ASSEMBLY [J].
AEBI, U ;
HANER, M ;
TRONCOSO, J ;
EICHNER, R ;
ENGEL, A .
PROTOPLASMA, 1988, 145 (2-3) :73-81
[3]  
ALBER T, 1987, PROTEIN ENG, P289
[4]   THE MOLECULAR-BIOLOGY OF INTERMEDIATE FILAMENT PROTEINS [J].
ALBERS, K ;
FUCHS, E .
INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1992, 134 :243-+
[5]   INTERMEDIATE FILAMENTS FORMED DENOVO FROM TAIL-LESS CYTOKERATINS IN THE CYTOPLASM AND IN THE NUCLEUS [J].
BADER, BL ;
MAGIN, TM ;
FREUDENMANN, M ;
STUMPP, S ;
FRANKE, WW .
JOURNAL OF CELL BIOLOGY, 1991, 115 (05) :1293-1307
[6]  
BARTNIK E, 1987, EUR J CELL BIOL, V44, P219
[7]   INTERMEDIATE FILAMENTS IN NON-NEURONAL CELLS OF INVERTEBRATES - ISOLATION AND BIOCHEMICAL-CHARACTERIZATION OF INTERMEDIATE FILAMENTS FROM THE ESOPHAGEAL EPITHELIUM OF THE MOLLUSK HELIX-POMATIA [J].
BARTNIK, E ;
OSBORN, M ;
WEBER, K .
JOURNAL OF CELL BIOLOGY, 1985, 101 (02) :427-440
[8]   ECTOPIC SYNTHESIS OF EPIDERMAL CYTOKERATINS IN PANCREATIC-ISLET CELLS OF TRANSGENIC MICE INTERFERES WITH CYTOSKELETAL ORDER AND INSULIN PRODUCTION [J].
BLESSING, M ;
RUTHER, U ;
FRANKE, WW .
JOURNAL OF CELL BIOLOGY, 1993, 120 (03) :743-755
[9]   NUCLEATED ASSEMBLY OF MICROTUBULES IN PORCINE BRAIN EXTRACTS [J].
BORISY, GG ;
OLMSTED, JB .
SCIENCE, 1972, 177 (4055) :1196-&
[10]  
BROWN DT, 1983, CELL TISSUE RES, V233, P619