CLONING AND CHARACTERIZATION OF CDNA CODING FOR A NEW ALLERGEN FROM THE HOUSE-DUST MITE, DERMATOPHAGOIDES-FARINAE

被引：34

作者：

AKI, T

ONO, K

PAIK, SY

WADA, T

JYO, T

SHIGETA, S

MUROOKA, Y

OKA, S

机构：

[1] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIGASHIHIROSHIMA 724,JAPAN

[2] FUMAKILLA LTD,DEV & ADM SECT,HIROSHIMA,JAPAN

[3] HIROSHIMA PREFECTURAL HOSP,DEPT INTERNAL MED 3,HIROSHIMA,JAPAN

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1994年 / 103卷 / 04期

关键词：

MITE ALLERGEN; CDNA; SEQUENCING; DERMATOPHAGOIDES FARINAE;

D O I：

10.1159/000236653

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A cDNA clone encoding a new allergen from the Dermatophagoides farinae cDNA lambda gt11 library was isolated and sequenced. There was no amino acid sequence homology with other known allergens. The gene product, beta-galactosidase fusion protein, of the truncated cDNA on blot reacted with IgE in 13 of 43 sera from patients allergic to mites. The affinity-purified fusion protein had a potent ability to release histamine from washed blood cells of the mite-allergic patients. Human specific IgE eluted from the fusion protein band on blots recognized a 39-kD component on blots of a mite body extract.

引用

页码：349 / 356

页数：8

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