EVANESCENT DETECTION OF ADSORBED PROTEIN CONCENTRATION-DISTANCE PROFILES - FIT OF SIMPLE-MODELS TO VARIABLE-ANGLE TOTAL INTERNAL-REFLECTION FLUORESCENCE DATA

Total internal reflection fluorescence spectroscopy (TIRF) is an established technique for following the course of interfacial reactions. Theoretically, by gathering TIRF data as a function of observation angle, one can obtain the density of fluorophores with respect to distance away from a solid/liquid interface. In order that the practical application of the theory might be explored, variable observation angle data from solutions of fluorescein and from adsorbed layers of fluorescein isothiocyanate labeled immunoglobulin have been analyzed in terms of simple concentration-distance profiles.