IDENTIFICATION OF DISULFIDE-LINKED PROTEIN COMPLEXES IN THE NUCLEOCAPSIDS OF HERPES-SIMPLEX VIRUS TYPE-2

被引：39

作者：

ZWEIG, M ^{[1
]}

HEILMAN, CJ ^{[1
]}

HAMPAR, B ^{[1
]}

机构：

[1] NCI,FREDERICK CANC RES CTR,MOLEC VIROL LAB,FREDERICK,MD 21701

来源：

VIROLOGY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1016/0042-6822(79)90474-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

High molecular weight disulfide-linked protein complexes in herpes simplex virus type 2 (HSV-2) nucleocapsids were demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis under nonreducing conditions. The appearance of these complexes was accompanied by a reduction in intensity of three major bands (p155, p50, and p40). The major disulfide-linked component (P350) was shown to contain p155 and p50 by diagonal gel electrophoresis. However, we were unable to resolve distinct disulfide-linked species possessing p40 by either SDS-PAGE or gel filtration in the presence of guanidine hydrochoride (GuHCl). © 1979.

引用

页码：442 / 450

页数：9

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